Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Inclusion body myositis laser microdissection reveals differential up-regulation of IFN-gamma signaling cascade in attacked versus nonattacked myofibers

Ivanidze, J., Hoffmann, R., Lochmueller, H., Engel, A. G., Hohlfeld, R., & Dornmair, K. (2011). Inclusion body myositis laser microdissection reveals differential up-regulation of IFN-gamma signaling cascade in attacked versus nonattacked myofibers. American Journal of Pathology, 179(3), 1347-1359. doi:10.1016/j.ajpath.2011.05.055.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Ivanidze, Jana1, Autor           
Hoffmann, Reinhard, Autor
Lochmueller, Hanns, Autor
Engel, Andrew G., Autor
Hohlfeld, Reinhard1, Autor           
Dornmair, Klaus1, Autor           
Affiliations:
1Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society, ou_1113547              

Inhalt

einblenden:
ausblenden:
Schlagwörter: IDIOPATHIC INFLAMMATORY MYOPATHIES; MHC CLASS-I; MONOCLONAL-ANTIBODY ANALYSIS; CELL-RECEPTOR EXPRESSION; SKELETAL-MUSCLE LEADS; CYTOTOXIC T-CELLS; COMPLEX CLASS-I; AUTOIMMUNE MYOSITIS; MONONUCLEAR-CELLS; KAPPA-BPathology;
 Zusammenfassung: Sporadic inclusion body myositis (IBM) is a muscle disease with two
separate pathogenic components, degeneration and inflammation.
Typically, nonnecrotic myofibers are focally surrounded and invaded by
CD8(+) T cells and macrophages. Both attacked and nonattacked myofibers
express high levels of human leukocyte antigen class I (HLA-I)
molecules, a prerequisite for antigen presentation to CD8(+) T cells.
However, only a subgroup of HLA-I+ myofibers is attacked by immune
cells. By using IHC, we classified myofibers from five patients with
sporadic IBM as attacked (A(IBM)) or nonattacked (N-IBM) and isolated
the intracellular contents of myofibers separately by laser
microdissection. For comparison, we isolated myofibers from control
persons (H-CTRL). The samples were analyzed by microarray hybridization
and quantitative PCR. HIA-I up-regulation was observed in A(IBM) and
N-IBM, whereas H-CTRL were negative for HLA-I. In contrast, the
inducible chain of the interferon (IFN) gamma receptor (IFNGR2) and
several IFN-gamma-induced genes were up-regulated in A(IBM) compared
with N-IBM and H-CTRL fibers. Confocal microscopy confirmed segmental
IFNGR2 up-regulation on the membranes of A(IBM), which positively
correlated with the number of adjacent CD8(+) T cells. Thus, the
differential up-regulation of the IFN-gamma signaling cascade observed
in the attacked fibers is related to local inflammation, whereas the
ubiquitous HLA-I expression on IBM muscle fibers does not require IFNGR
expression. (Am J Pathol 2011, 179:1347-1359;
DOI:10.1016/j.ajpath.2011.05.055)

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2011-09
 Publikationsstatus: Erschienen
 Seiten: 13
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000298307300029
DOI: 10.1016/j.ajpath.2011.05.055
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: American Journal of Pathology
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Hagerstown, Md., etc. : American Association of Pathologists and Bacteriologists [etc.]
Seiten: - Band / Heft: 179 (3) Artikelnummer: - Start- / Endseite: 1347 - 1359 Identifikator: ISSN: 0002-9440
CoNE: https://pure.mpg.de/cone/journals/resource/954925377902