ausblenden:
Schlagwörter:
Transgenic plants
Real-time pcr
Copy number
Zygosity.
Polymerase-chain-reaction
Agrobacterium-mediated transformation
Event-specific detection
Quantitative pcr
Detection system
Competitive pcr
Taqman assay
Rapid method
Green-i
Expression.
Plant Sciences in Current Contents(R)/Agricultural, Biology &
Environmental Sciences. Animal & Plant Sciences in Current
Contents(R)/Life Sciences.
Zusammenfassung:
This review examines how real-time PCR can be used to determine copy number and zygosity in transgenic plants. Distinguishing between plants that harbor one and two copies of a transgene or are hemizygous and homozygous requires the ability to routinely