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  Collision Cross Sections and Ion Mobility Separation of Fragment Ions from Complex N-Glycans

Harvey, D. J., Watanabe, Y., Allen, J. D., Rudd, P., Pagel, K., Crispin, M., et al. (2018). Collision Cross Sections and Ion Mobility Separation of Fragment Ions from Complex N-Glycans. Journal of the American Society for Mass Spectrometry. doi:10.1007/s13361-018-1930-1.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-758A-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-758B-F
Genre: Journal Article

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 Creators:
Harvey, David J.1, 2, Author
Watanabe, Yasunori2, 3, 4, Author
Allen, Joel D.2, Author
Rudd, Pauline5, Author
Pagel, Kevin6, 7, Author              
Crispin, Max2, Author
Struwe, Weston B.3, 8, Author
Affiliations:
1Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7FZ, UK, escidoc:persistent22              
2Biological Sciences and the Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK, escidoc:persistent22              
3Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK, escidoc:persistent22              
4Division of Structural Biology, University of Oxford, Wellcome Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, UK, escidoc:persistent22              
5NIBRT GlycoScience Group, The National Institute for Bioprocessing Research and Training, Dublin, Ireland, escidoc:persistent22              
6Molecular Physics, Fritz Haber Institute, Max Planck Society, escidoc:634545              
7Institut für Chemie und Biochemie, Freien Universität BerlinTakustrasse 3, 14195, Berlin, Germany, escidoc:persistent22              
8Chemistry Research Laboratory, Department of Chemistry, University of Oxford, South Parks Road, Oxford, OX1 3TA, UK, escidoc:persistent22              

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Free keywords: Ion mobility, Complex N-glycans, Collision cross section, Glycomics
 Abstract: Ion mobility mass spectrometry (IM- MS) holds great potential for structural glycobiology, in particular in its ability to resolve glycan isomers. Generally, IM-MS has largely been applied to intact glycoconjugate ions with reports focusing on the separation of different adduct types. Here, we explore IM separation and report the collision cross section (CCS) of complex type N-glycans and their fragments in negative ion mode following collision-induced dis- sociation (CID). CCSs of isomeric fragment ions were found, in some cases, to reveal structural details that were not present in CID spectra themselves. Many fragment ions were confirmed as possessing multiple structure, details of which could be obtained by comparing their drift time profiles to different glycans. By using fragmen- tation both before and after mobility separation, information was gathered on the fragmentation pathways producing some of the ions. These results help demonstrate the utility of IM and will contribute to the growing use of IM-MS for glycomics.

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Language(s): eng - English
 Dates: 2017-12-152018-02-272018-04-19
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s13361-018-1930-1
 Degree: -

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Title: Journal of the American Society for Mass Spectrometry
Source Genre: Journal
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Publ. Info: New York, NY : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1044-0305
CoNE: http://pubman.mpdl.mpg.de/cone/journals/resource/954925590403