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  Sub-100-nm metafluorophores with digitally tunable optical properties self-assembled from DNA

Woehrstein, J. B., Strauss, M. T., Ong, L. L., Wei, B., Zhang, D. Y., Jungmann, R., et al. (2017). Sub-100-nm metafluorophores with digitally tunable optical properties self-assembled from DNA. Science Advances, 3(6): e1602128. doi:10.1126/sciadv.1602128.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-E5EC-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-E5ED-F
Genre: Journal Article

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e1602128.full.pdf (Publisher version), 2MB
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© 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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 Creators:
Woehrstein, Johannes B.1, Author              
Strauss, Maximilian T.1, Author              
Ong, Luvena L.2, Author
Wei, Bryan2, Author
Zhang, David Y.2, Author
Jungmann, Ralf1, Author              
Yin, Peng2, Author
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, escidoc:2149679              
2external, escidoc:persistent22              

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Free keywords: HYBRIDIZATION CHAIN-REACTION; IN-SITU HYBRIDIZATION; SINGLE-MOLECULE; SUPERRESOLUTION MICROSCOPY; FLUORESCENCE MICROSCOPY; GENE-EXPRESSION; ORIGAMI; PROTEIN; NANOTECHNOLOGY; BARCODESScience & Technology - Other Topics;
 Abstract: Fluorescence microscopy allows specific target detection down to the level of single molecules and has become an enabling tool in biological research. To transduce the biological information to an imageable signal, we have developed a variety of fluorescent probes, such as organic dyes or fluorescent proteins with different colors. Despite their success, a limitation on constructing small fluorescent probes is the lack of a general framework to achieve precise and programmable control of critical optical properties, such as color and brightness. To address this challenge, we introduce metafluorophores, which are constructed as DNA nanostructure-based fluorescent probes with digitally tunable optical properties. Each metafluorophore is composed of multiple organic fluorophores, organized in a spatially controlled fashion in a compact sub-100-nm architecture using a DNA nanostructure scaffold. Using DNA origami with a size of 90 x 60 nm(2), substantially smaller than the optical diffraction limit, we constructed small fluorescent probes with digitally tunable brightness, color, and photostability and demonstrated a palette of 124 virtual colors. Using these probes as fluorescent barcodes, we implemented an assay for multiplexed quantification of nucleic acids. Additionally, we demonstrated the triggered in situ self-assembly of fluorescent DNA nanostructures with prescribed brightness upon initial hybridization to a nucleic acid target.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000406370700008
DOI: 10.1126/sciadv.1602128
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Title: Science Advances
  Other : Sci. Adv.
Source Genre: Journal
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Publ. Info: Washington : AAAS
Pages: - Volume / Issue: 3 (6) Sequence Number: e1602128 Start / End Page: - Identifier: ISSN: 2375-2548
CoNE: http://pubman.mpdl.mpg.de/cone/journals/resource/2375-2548