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  Sub-100-nm metafluorophores with digitally tunable optical properties self-assembled from DNA

Woehrstein, J. B., Strauss, M. T., Ong, L. L., Wei, B., Zhang, D. Y., Jungmann, R., et al. (2017). Sub-100-nm metafluorophores with digitally tunable optical properties self-assembled from DNA. Science Advances, 3(6): e1602128. doi:10.1126/sciadv.1602128.

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© 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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http://advances.sciencemag.org/cgi/content/full/3/6/e1602128/DC1 (Ergänzendes Material)
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 Urheber:
Woehrstein, Johannes B.1, Autor           
Strauss, Maximilian T.1, Autor           
Ong, Luvena L.2, Autor
Wei, Bryan2, Autor
Zhang, David Y.2, Autor
Jungmann, Ralf1, Autor           
Yin, Peng2, Autor
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              
2external, ou_persistent22              

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Schlagwörter: HYBRIDIZATION CHAIN-REACTION; IN-SITU HYBRIDIZATION; SINGLE-MOLECULE; SUPERRESOLUTION MICROSCOPY; FLUORESCENCE MICROSCOPY; GENE-EXPRESSION; ORIGAMI; PROTEIN; NANOTECHNOLOGY; BARCODESScience & Technology - Other Topics;
 Zusammenfassung: Fluorescence microscopy allows specific target detection down to the level of single molecules and has become an enabling tool in biological research. To transduce the biological information to an imageable signal, we have developed a variety of fluorescent probes, such as organic dyes or fluorescent proteins with different colors. Despite their success, a limitation on constructing small fluorescent probes is the lack of a general framework to achieve precise and programmable control of critical optical properties, such as color and brightness. To address this challenge, we introduce metafluorophores, which are constructed as DNA nanostructure-based fluorescent probes with digitally tunable optical properties. Each metafluorophore is composed of multiple organic fluorophores, organized in a spatially controlled fashion in a compact sub-100-nm architecture using a DNA nanostructure scaffold. Using DNA origami with a size of 90 x 60 nm(2), substantially smaller than the optical diffraction limit, we constructed small fluorescent probes with digitally tunable brightness, color, and photostability and demonstrated a palette of 124 virtual colors. Using these probes as fluorescent barcodes, we implemented an assay for multiplexed quantification of nucleic acids. Additionally, we demonstrated the triggered in situ self-assembly of fluorescent DNA nanostructures with prescribed brightness upon initial hybridization to a nucleic acid target.

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Sprache(n): eng - English
 Datum: 2017
 Publikationsstatus: Online veröffentlicht
 Seiten: 12
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000406370700008
DOI: 10.1126/sciadv.1602128
 Art des Abschluß: -

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Titel: Science Advances
  Andere : Sci. Adv.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Washington : AAAS
Seiten: - Band / Heft: 3 (6) Artikelnummer: e1602128 Start- / Endseite: - Identifikator: ISSN: 2375-2548
CoNE: https://pure.mpg.de/cone/journals/resource/2375-2548