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  Analyzing the mesoscopic structure of pericellular coats on living cells

Böhm, H., Mundinger, T. A., Hagel, V., Boehm, C. H. J., Curtis, J. E., & Spatz, J. P. (2010). Analyzing the mesoscopic structure of pericellular coats on living cells. In M. J. Buehler, D. Kaplan, C. T. Lim, & J. Spatz (Eds.), Biological Materials and Structures in Physiologically Extreme Conditions and Disease (pp. 19-24). Warrendale, PA, USA: MRS. Retrieved from https://www.cambridge.org/core/journals/mrs-online-proceedings-library-archive/article/div-classtitleanalyzing-the-mesoscopic-structure-of-pericellular-coats-on-living-cellsdiv/4A98A2AB6F3579E1CCA54F68C21F10BC.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-3C47-4 Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-ABD5-7
Genre: Konferenzbeitrag

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externe Referenz:
https://doi.org/10.1557/PROC-1274-QQ02-03 (beliebiger Volltext)
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Urheber

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 Urheber:
Böhm, Heike1, Autor           
Mundinger, Tabea A, Autor
Hagel, Valentin, Autor
Boehm, Christian H. J., Autor
Curtis, Jennifer E, Autor
Spatz, Joachim P.1, Autor           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Schlagwörter: -
 Zusammenfassung: We employed passive particle-tracking microrheology to map the micromechanical structure of the hyaluronan-rich pericellular coat enveloping chondrocytes. Therefor we exploited the technique's position sensitivity to gain radial information on the coat. We observed a linear increase in viscoelasticity from the coat's rim towards the cell membrane. This gradient corresponds to hyaluronan concentration profiles observed in confocal fluorescent microscopy with small, specific hyaluronan markers. These results suggest that the structural basis of the pericellular coat is formed by grafted hyaluronan of different effective lengths stretched out by a homogenous decoration with hyaladherins such as aggrecan. The different effective lengths could be caused either by different lengths of the HA chains or by “side-on” attachments within the chain. Remarkably, the hyaluronan-rich coat increases the viscosity of the pericellular space only by about a factor of about two at 100 and at 20 Hz compared to pure media and an increasing elastic component is observed. Both the viscoelasticity as well as the hyaluronan concentration decrease linearly or slightly exponential from the cell membrane towards the PCC's rim. These observations could be obtained on living cells exploiting this unintrusive measurement techniques.

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Sprache(n): eng - English
 Datum: 2011-02-012011-02-012010
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 522155
URI: https://www.cambridge.org/core/journals/mrs-online-proceedings-library-archive/article/div-classtitleanalyzing-the-mesoscopic-structure-of-pericellular-coats-on-living-cellsdiv/4A98A2AB6F3579E1CCA54F68C21F10BC
 Art des Abschluß: -

Veranstaltung

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Titel: MRS
Veranstaltungsort: San Francisco, CA, USA
Start-/Enddatum: 2010-04-05 - 2010-04-09

Entscheidung

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Projektinformation

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Quelle 1

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Titel: Biological Materials and Structures in Physiologically Extreme Conditions and Disease
Genre der Quelle: Konferenzband
 Urheber:
Buehler, M. J.1, Herausgeber           
Kaplan, D., Herausgeber
Lim, C. T., Herausgeber
Spatz, J.2, Herausgeber           
Affiliations:
1 Former Dept. Theory of Mesoscopic Phenomena, Max Planck Institute for Intelligent Systems, Max Planck Society, ou_1497656            
2 Dept. New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Max Planck Society, ou_1497649            
Ort, Verlag, Ausgabe: Warrendale, PA, USA : MRS
Seiten: - Band / Heft: - Artikelnummer: - Start- / Endseite: 19 - 24 Identifikator: -

Quelle 2

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Titel: MRS Symposium Proceedings
Genre der Quelle: Reihe
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 1274 Artikelnummer: 1274-QQ02-03 Start- / Endseite: - Identifikator: -