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  Challenges in imaging cell surface receptor clusters

Medda, R., Giske, A., & Cavalcanti-Adam, E. A. (2016). Challenges in imaging cell surface receptor clusters. Optics and Lasers in Engineering, 76, 3-8. doi:10.1016/j.optlaseng.2015.03.020.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-ACEB-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-C863-0
Genre: Journal Article

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OptLaserEng_76_2016_3.pdf (Any fulltext), 3MB
 
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 Creators:
Medda, Rebecca1, Author              
Giske, Arnold, Author
Cavalcanti-Adam, Elisabetta Ada1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, escidoc:2364731              

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Free keywords: BMP receptors; STED microscopy; Super-resolution microscopy; Fluorescence microscopy
 Abstract: Super-resolution microscopy offers unique tools for visualizing and resolving cellular structures at the molecular level. STED microscopy is a purely optical method where neither complex sample preparation nor mathematical post-processing is required. Here we present the use of STED microscopy for imaging receptor cluster composition. We use two-color STED to further determine the distribution of two different receptor subunits of the family of receptor serine/threonine kinases in the presence or absence of their ligands. The implications of receptor clustering on the downstream signaling are discussed, and future challenges are also presented.

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Language(s): eng - English
 Dates: 2015-03-172014-10-132015-03-242015-04-142016-01-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.optlaseng.2015.03.020
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Title: Optics and Lasers in Engineering
  Other : Opt. Lasers Eng.
Source Genre: Journal
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Publ. Info: London : Elsevier
Pages: - Volume / Issue: 76 Sequence Number: - Start / End Page: 3 - 8 Identifier: ISSN: 0143-8166
CoNE: http://pubman.mpdl.mpg.de/cone/journals/resource/954925472383