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  The Impact of High-Fat Diet on Metabolism and Immune Defense in Small Intestine Mucosa

Wisniewski, J. R., Friedrich, A., Keller, T., Mann, M., & Koepsell, H. (2015). The Impact of High-Fat Diet on Metabolism and Immune Defense in Small Intestine Mucosa. JOURNAL OF PROTEOME RESEARCH, 14(1), 353-365. doi:10.1021/pr500833v.

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Wisniewski, Jacek R.1, Autor           
Friedrich, Alexandra2, Autor
Keller, Thorsten2, Autor
Mann, Matthias1, Autor           
Koepsell, Hermann2, Autor
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

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Schlagwörter: INFLAMMATORY-BOWEL-DISEASE; CANCER RESISTANCE PROTEIN; ENTEROCYTE BRUSH-BORDER; TRANSPORTERS; TOLERANCE; MEMBRANE; COMBINATION; ABSORPTION; FAMILY; TISSUESmall intestine; intestinal pithelium; proteomics; nutrition; high-fat diet; intestinal metabolism; nutrient transport; drug transport; immune defense; major histocompatibility complex (MHC);
 Zusammenfassung: Improved procedures for sample preparation and proteomic data analysis allowed us to identify 7700 different proteins in mouse small intestinal mucosa and calculate the concentrations of >5000 proteins. We compared protein concentrations of small intestinal mucosa from mice that were fed for two months with normal diet (ND) containing 34.4% carbohydrates, 19.6% protein, and 3.3% fat or high-fat diet (HFD) containing 25.3% carbohydrates, 24.1% protein, and 34.6% fat. Eleven percent of the quantified proteins were significantly different between ND and HFD. After HFD, we observed an elevation of proteins involved in protein synthesis, protein N-glycosylation, and vesicle trafficking. Proteins engaged in fatty acid absorption, fatty acid beta-oxidation, and steroid metabolism were also increased. Enzymes of glycolysis and pentose phosphate cycle were decreased, whereas proteins of the respiratory chain and of ATP synthase were increased. The protein concentrations of various nutrient transporters located in the enterocyte plasma membrane including the Na+-d-glucose cotransporter SGLT1, the passive glucose transporter GLUT2, and the H+-peptide cotransporter PEPT1 were decreased. The concentration of the Na+,K+-ATPase, which turned out to be the most strongly expressed enterocyte transporter, was also decreased. HFD also induced concentration changes of drug transporters and of enzymes involved in drug metabolism, which suggests effects of HFD on pharmacokinetics and toxicities. Finally, we observed down-regulation of antibody subunits and of components of the major histocompatibility complex II that may reflect impaired immune defense and immune tolerance in HFD. Our work shows dramatic changes in functional proteins of small intestine mucosa upon excessive fat consumption.

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Sprache(n): eng - English
 Datum: 2014-102015-01
 Publikationsstatus: Erschienen
 Seiten: 13
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000347506600030
DOI: 10.1021/pr500833v
 Art des Abschluß: -

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Titel: JOURNAL OF PROTEOME RESEARCH
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 1155 16TH ST, NW, WASHINGTON, DC 20036 USA : AMER CHEMICAL SOC
Seiten: - Band / Heft: 14 (1) Artikelnummer: - Start- / Endseite: 353 - 365 Identifikator: ISSN: 1535-3893