hide
Free keywords:
Acetylation
Arabidopsis/*cytology/drug effects/growth & development
Arabidopsis Proteins/metabolism
Cell Division/drug effects
Cell Proliferation/drug effects
Cell Wall/drug effects
Histone Deacetylase Inhibitors/*pharmacology
Histones/metabolism
Hydroxamic Acids/*pharmacology
Indoleacetic Acids/metabolism
Pollen/cytology/drug effects/growth & development
Signal Transduction/drug effects
Abstract:
The haploid male gametophyte, the pollen grain, is a terminally differentiated structure whose function ends at fertilization. Plant breeding and propagation widely use haploid embryo production from in vitro-cultured male gametophytes, but this technique remains poorly understood at the mechanistic level. Here, we show that histone deacetylases (HDACs) regulate the switch to haploid embryogenesis. Blocking HDAC activity with trichostatin A (TSA) in cultured male gametophytes of Brassica napus leads to a large increase in the proportion of cells that switch from pollen to embryogenic growth. Embryogenic growth is enhanced by, but not dependent on, the high-temperature stress that is normally used to induce haploid embryogenesis in B. napus. The male gametophyte of Arabidopsis thaliana, which is recalcitrant to haploid embryo development in culture, also forms embryogenic cell clusters after TSA treatment. Genetic analysis suggests that the HDAC protein HDA17 plays a role in this process. TSA treatment of male gametophytes is associated with the hyperacetylation of histones H3 and H4. We propose that the totipotency of the male gametophyte is kept in check by an HDAC-dependent mechanism and that the stress treatments used to induce haploid embryo development in culture impinge on this HDAC-dependent pathway.
