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  Miniaturization of two-photon microscopy for imaging in freely moving animals

Helmchen, F., Denk, W., & Kerr, J. (2013). Miniaturization of two-photon microscopy for imaging in freely moving animals. Cold Spring Harbor Protocols, 2013(10), 904-913. doi:10.1101/pdb.top078147.

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Helmchen, Fritjof1, Author           
Denk, Winfried2, Author           
Kerr, Jason2, Author           
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1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: This article describes the development and application of miniaturized two-photon-excited fluorescence microscopes (“two-photon fiberscopes”). Two-photon fiberscopes have been developed with the aim of enabling high-resolution imaging of neural activity in freely behaving animals. They use fiber optics to deliver laser light for two-photon excitation. Their small front piece typically contains a miniature scanning mechanism and imaging optics. Two-photon fiberscopes can be made sufficiently small and lightweight to be carried by rats and mice and to allow virtually unrestricted movement within a behavioral arena. Typically mounted to the animal’s skull above a cranial window, two-photon fiberscopes permit imaging of cells down to at least 250 µm below the brain surface (e.g., in rat neocortex). In freely exploring animals, action-potential-evoked calcium transients can be imaged in individual somata of visual cortex neurons bulk-labeled with a calcium indicator. Two-photon fiberscopes thus enable high-resolution optical recording of neural activity with cellular resolution during natural behaviors

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Language(s): eng - English
 Dates: 2013
 Publication Status: Issued
 Pages: 10
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 Rev. Type: Peer
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Title: Cold Spring Harbor Protocols
Source Genre: Journal
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Publ. Info: NY : Cold Spring Harbor Laboratory Press
Pages: - Volume / Issue: 2013 (10) Sequence Number: - Start / End Page: 904 - 913 Identifier: ISSN: 1940-3402