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  A combined solution exchange/plunge-freezing device for skinned muscle fibers

Stegmann, H., & Fink, R. H. A. (1999). A combined solution exchange/plunge-freezing device for skinned muscle fibers. Journal of Muscle Research and Cell Motility, 20(5), 497-503. doi:10.1023/A:1005527328882.

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Genre: Journal Article
Alternative Title : A combined solution exchange/plunge-freezing device for skinned muscle fibers

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JMuscleResCellMotil_20_1999_497.pdf (Any fulltext), 277KB
 
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 Creators:
Stegmann, Heiko1, Author           
Fink, R. H. A., Author
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1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: For many contractility studies, defined functional states of skinned muscle fiber preparations can be introduced by application of standardized perfusion protocols with large varieties of experimental solutions. Functionally important subcellular element distributions in the myoplasm and in the sarcoplasmic reticulum can be measured with high spatial resolution by electron microscopic microanalysis. Capturing these subcellular ion distributions requires their rapid immobilization by quick-freezing. We therefore combined a plunge-freezing device with a semiautomatic solution exchanger to reproducibly perfuse skinned muscle fiber bundles with multiple solutions. The isometric tension produced is simultaneously recorded as an indicator for the functional state. The samples can be quick-frozen at any chosen time of the tension transient. A cryoglueing technique finally delivers specimens suitable for cryoultramicrotomy

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Language(s): eng - English
 Dates: 1999-04-031999-05-031999
 Publication Status: Issued
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Journal of Muscle Research and Cell Motility
  Other : J. Muscle Res. Cell Motil.
Source Genre: Journal
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Publ. Info: London : Chapman and Hall
Pages: - Volume / Issue: 20 (5) Sequence Number: - Start / End Page: 497 - 503 Identifier: ISSN: 0142-4319
CoNE: https://pure.mpg.de/cone/journals/resource/954925472360