Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  Determination of styrene oxide adducts in DNA and DNA components

Schrader, W., & Linscheidt, M. (1995). Determination of styrene oxide adducts in DNA and DNA components. Journal of Chromatography A, 717(1-2), 117-125. doi:10.1016/0021-9673(95)00558-9.

Item is

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Schrader, W.1, 2, Autor           
Linscheidt, M.1, Autor
Affiliations:
1AS, Institut für Spektrochemie und Angewandte Spektroskopie, P.O. Box 101352, D-44013 Dortmund, Germany, ou_persistent22              
2Service Department Schrader (MS), Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445629              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: The reaction of styrene oxide with DNA components was studied using separation by capillary zone electrophoresis (CZE) and detection by negative-ion electrospray mass spectrometry (MS). The CZE-MS interface was built for a sector field mass spectrometer. The reaction of styrene oxide with mononucleotides (dGMP, dAMP) was used to optimize the relevant separation parameters and to gather the first information about the behaviour of the possible products. With these mixtures, sample stacking procedures were developed and the scope of collision-induced dissociations were studied. From the fragments recorded, information about the reaction sites in the nucleotides was obtained. Further, the reaction with intact calf thymus DNA was investigated. The DNA was digested into oligonucleotides using the previously described approach with Benzonase, an unspecific nuclease, and alkaline phosphatase. Styrene oxide mono-adducts in dinucleotides, trinucleotides and tetranucleotides were detected, whereas pentanucleotides exhibit mono- and discernible amounts of di-adducts. The hexanucleotides were generally modified twice. The alkylated species moved faster than the unmodified oligomers.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 1998-09-031995-11-24
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/0021-9673(95)00558-9
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Journal of Chromatography A
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Amsterdam : Elsevier
Seiten: - Band / Heft: 717 (1-2) Artikelnummer: - Start- / Endseite: 117 - 125 Identifikator: ISSN: 0021-9673
CoNE: https://pure.mpg.de/cone/journals/resource/954925527837_1