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  Aldol Sensors for the Rapid Generation of Tunable Fluorescence by Antibody Catalysis

List, B., Barbas, C., & Lerner, R. (1998). Aldol Sensors for the Rapid Generation of Tunable Fluorescence by Antibody Catalysis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 95(26), 15351-15355. doi:10.1073/pnas.95.26.15351.

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List, Benjamin1, 2, Author           
Barbas, CF2, Author
Lerner, RA2, Author
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1Research Department List, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445585              
2Scripps Res Inst, Skaggs Inst Chem Biol, 10550 N Torrey Pines Rd,BCC 515, La Jolla, CA 92037 , ou_persistent22              

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 Abstract: The synthesis of novel fluorogenic retro-aldol substrates for aldolase antibody 38C2 is described. These substrates are efficiently and specifically processed by antibody aldolases but not by natural cellular enzymes. Together, the fluorogenic substrates and antibody aldolases provide reporter gene systems that are compatible with living cells. The broad scope of the antibody aldolase allows for the processing of a range of substrates that can be designed to allow fluorescence monitoring at a variety of wavelengths. We also have developed the following concept in fluorescent protein tags. beta-Diketones bearing a fluorescent tag are bound Synthesis of the Aldol Sensors covalently by the aldolase antibody and not other proteins. We anticipate that proteins fused with the antibody can be tagged specifically and covalently within living cells with fluorophores of virtually any color, thereby providing an alternative to green fluorescent protein fusions.

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 Dates: 1998-12-22
 Publication Status: Issued
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 Identifiers: DOI: 10.1073/pnas.95.26.15351
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Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Source Genre: Journal
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Pages: - Volume / Issue: 95 (26) Sequence Number: - Start / End Page: 15351 - 15355 Identifier: -