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  Free-flow electrophoresis in proteome sample preparation

Wildgruber, R., Weber, G., Wise, P., Grimm, D., & Bauer, J. (2014). Free-flow electrophoresis in proteome sample preparation. PROTEOMICS, 14(4-5), 629-636. doi:10.1002/pmic.201300253.

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 Urheber:
Wildgruber, Robert1, Autor
Weber, Gerhard1, Autor
Wise, Petra1, Autor
Grimm, Daniela1, Autor
Bauer, Johann2, Autor           
Affiliations:
1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Schlagwörter: TANDEM MASS-SPECTROMETRY; THYROID-CELL LINES; MEMBRANE-PROTEINS; PLANT-MITOCHONDRIA; ENDOTHELIAL-CELLS; SODIUM-CHLORIDE; SURFACE-CHARGE; SEPARATION; PLASMA; PURIFICATIONCell biology; Isoelectric point; Matrix free; Protein complex; Subcellular particles;
 Zusammenfassung: An aim of proteome research is to identify the entire complement of proteins expressed in defined cell types of humans, animals, plants, and microorganisms. The approach requires searching for low abundant or even rarely expressed proteins in many cell types, as well as the determination of the protein expression levels in subcellular compartments and organelles. In recent years, rather powerful MS technologies have been developed. At this stage of MS device development, it is of highest interest to purify intact cell types or isolate subcellular compartments, where the proteins of interest are originating from, which determine the final composition of a peptide mixture. Free-flow electrophoresis proved to be useful to prepare meaningful peptide mixtures because of its improved capabilities in particle electrophoresis and the enhanced resolution in protein separation. Sample preparation by free-flow electrophoresis mediated particle separation was preferentially performed for purification of either organelles and their subspecies or major protein complexes. Especially, the introduction of isotachophoresis and interval zone electrophoresis improved the purity of the gained analytes of interest. In addition, free-flow IEF proved to be helpful, when proteins of low solubility, obtained, e.g. from cell membranes, were investigated.

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Sprache(n): eng - English
 Datum: 2014-03
 Publikationsstatus: Erschienen
 Seiten: 8
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000332341200023
DOI: 10.1002/pmic.201300253
 Art des Abschluß: -

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Titel: PROTEOMICS
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Seiten: - Band / Heft: 14 (4-5) Artikelnummer: - Start- / Endseite: 629 - 636 Identifikator: ISSN: 1615-9853