Fluorescence Studies on N−(3−Pyrene)Maleinimide−Labeled Sarcoplasmic Reticulum 
ATPase in Native and Solubilized Membranes

Lüdi, Hans; Hasselbach, Wilhelm

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Fluorescence Studies on N−(3−Pyrene)Maleinimide−Labeled Sarcoplasmic Reticulum ATPase in Native and Solubilized Membranes

Lüdi, H., & Hasselbach, W. (1982). Fluorescence Studies on N−(3−Pyrene)Maleinimide−Labeled Sarcoplasmic Reticulum ATPase in Native and Solubilized Membranes. Zeitschrift fuer Naturforschung C, 37(1982), 1170-1179. Retrieved from http://www.mendeley.com/research/fluorescence-studies-on-n3pyrenemaleinimidelabeled-sarcoplasmic-reticulum-atpase-in-native-and-solubilized-membranes/.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0019-B009-F Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0019-B00A-D

Genre: Journal Article

Alternative Title : Fluorescence Studies on N−(3−Pyrene)Maleinimide−Labeled Sarcoplasmic Reticulum ATPase in Native and Solubilized Membranes

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Lüdi, Hans, Author
Hasselbach, Wilhelm¹, Author

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1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712

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Abstract: Fluorescence polarization and formation of excimers were studied in N−(3−pyrene)maleinimide−labeled sarcoplasmic reticulum vesicles. 1. The polarization of pyrenemaleinimide labeled vesicles does not change with temperature and shows a pronounced decrease at labeling concentrations larger than 1 mol pyrenemaleinimide per 10 mol ATPase. 2. Solubilization of the membrane with renders the polarization temperature dependent, but does not affect the concentration dependent depolarization observed in native vesicles. 3. The polarization of labeled vesicles is much smaller than to be expected from the temperature independent polarization indicating that the pyrenemaleinimide polarization did not monitor the rotation of the entire ATPase. Thus segmental motion occurs. 4. Pyrene excimers are observed at label concentrations larger than 1 mol label per 2.5 mol ATPase. 5. The amount of excimers was critically dependent on added detergents. From the fact that non−solubilizing amounts of strongly reduced the amount of pyrene excimers it is concluded that in the native sarcoplasmic reticulum vesicles at least two ATPase molecules must be in close contact

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Language(s): eng - English

Dates: Submitted: 1982-08-16Date issued: 1982

Publication Status: Issued

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Identifiers: eDoc: 664627
URI: http://www.mendeley.com/research/fluorescence-studies-on-n3pyrenemaleinimidelabeled-sarcoplasmic-reticulum-atpase-in-native-and-solubilized-membranes/
Other: 7547

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Title: Zeitschrift fuer Naturforschung C

Alternative Title : Z. Naturforsch. C

Source Genre: Journal

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Pages: - Volume / Issue: 37 (1982) Sequence Number: - Start / End Page: 1170 - 1179 Identifier: -