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  A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system

Edwards, F. A., Konnerth, A., Sakmann, B., & Takahashi, T. (1989). A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system. Pfluegers Archiv European Journal of Physiology, 414(5), 600-612. doi:10.1007/BF00580998.

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 Urheber:
Edwards, Francis A., Autor
Konnerth, Arthur, Autor
Sakmann, Bert1, Autor           
Takahashi, Tomoyuki, Autor
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

Inhalt

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Schlagwörter: Patch clamp; Brain slice; Central nervous system
 Zusammenfassung: (1) A preparation is described which allows patch clamp recordings to be made on mammalian central nervous system (CNS) neurones in situ. (2) A vibrating tissue slicer was used to cut thin slices in which individual neurones could be identified visually. Localized cleaning of cell somata with physiological saline freed the cell membrane, allowing the formation of a high resistance seal between the membrane and the patch pipette. (3) The various configurations of the patch clamp technique were used to demonstrate recording of membrane potential, whole cell currents and single channel currents from neurones and isolated patches. (4) The patch clamp technique was used to record from neurones filled with fluorescent dyes. Staining was achieved by filling cells during recording or by previous retrograde labelling. (5) Thin slice cleaning and patch clamp techniques were shown to be applicable to the spinal cord and almost any brain region and to various species. These techniques are also applicable to animals of a wide variety of postnatal ages, from newborn to adult.

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Sprache(n): eng - English
 Datum: 1989-01-311988-11-071989-02-141989-09
 Publikationsstatus: Erschienen
 Seiten: 13
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Art des Abschluß: -

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Titel: Pfluegers Archiv European Journal of Physiology
  Alternativer Titel : Pfluegers Arch.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Berlin ; Heidelberg : Springer
Seiten: - Band / Heft: 414 (5) Artikelnummer: - Start- / Endseite: 600 - 612 Identifikator: ISSN: 1432-2013