English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Crystal structure of the breakpoint cluster region homology domain from phosphoinositide 3-kinase p85 alpha subunit

Musacchio, A., Cantley, L. C., & Harrison, S. C. (1996). Crystal structure of the breakpoint cluster region homology domain from phosphoinositide 3-kinase p85 alpha subunit. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 93(25), 14373-14378. doi:10.1073/pnas.93.25.14373.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Musacchio, Andrea1, Author           
Cantley, Lewis C.2, Author
Harrison, Stephen C.3, Author
Affiliations:
1Abt. I:Mechanistische Zellbiologie, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753287              
2Cell Biology, Harvard Medical School, Boston, MA 02115, USA, ou_persistent22              
3Laboratory of Molecular Medicine and ¶ Howard Hughes Medical Institute, Children’s Hospital, 300 Longwood Avenue, Boston, MA 02115, USA, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Proteins such as the product of the breakpoint cluster region, chimaerin, and the Src homology 3-binding protein 3BP1, are GTPase activating proteins (GAPs) for members of the Rho subfamily of small GTP-binding proteins (G proteins or GTPases). A 200-residue region, named the breakpoint cluster region homology (BH) domain, is responsible for the GAP activity. We describe here the crystal structure of the BH domain from the p85 subunit of phosphatidylinositol 3-kinase at 2.0 Angstrom resolution. The domain is composed of seven helices, having a previously unobserved arrangement. A core of four helices contains most residues that are conserved in the BH family. Their packing suggests the location of a G-protein binding site. This structure of a GAP-like domain for small GTP-binding proteins provides a framework for analyzing the function of this class of molecules.

Details

show
hide
Language(s):
 Dates: 1996
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: A1996VY44800035
DOI: 10.1073/pnas.93.25.14373
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 93 (25) Sequence Number: - Start / End Page: 14373 - 14378 Identifier: ISSN: 0027-8424