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Free keywords:
HEPTYL ESTERS; REMOVAL
Abstract:
New methods are reported to establish enzymatic techniques for the chemo- and regioselective functionalization of carbohydrates, peptides and glycoconjugates. The N-terminal deprotection of peptides is achieved via hydrolysis of phenylacetamides, employing penicillin G acylase as biocatalyst. The C-terminal carboxyl function of peptides and O-glycopeptides is liberated by the lipase-mediated hydrolysis of heptyl esters. Penicillin G acylase and citrus acetylesterase are used to effect the chemo- and regioselective removal of phenylacetyl and acetyl blocking groups, respectively from differently protected carbohydrates.
