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SOLID-PHASE SYNTHESIS; PHOSPHOTYROSYL PEPTIDE; PROTECTING GROUPS; PHOSPHORYLATION; PROTEIN; SERINE; RECEPTOR; SIGNAL; RAS
Abstract:
Base-labile biologically relevant phosphopeptides can advantageously be synthesized under very mild conditions (pH 7, 37 degrees C) by employing the heptyl (Hep) ester as enzyme-labile protecting group. This method was used in the construction of a characteristic, selectively phosphorylated hydroxyamino acid fragment of the Raf-l kinase, an important constituent of the Ras pathway of signal transduction. (C) 1997 Elsevier Science Ltd.