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  Selective enzymatic removal of protecting groups from phosphopeptides: Chemoenzymatic synthesis of a characteristic phosphopeptide fragment of the Raf-1 kinase

Sebastian, D., Heuser, A., Schulze, S., & Waldmann, H. (1997). Selective enzymatic removal of protecting groups from phosphopeptides: Chemoenzymatic synthesis of a characteristic phosphopeptide fragment of the Raf-1 kinase. Synthesis, 1997(9), 1098-1108.

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 Creators:
Sebastian, D1, Author
Heuser, A1, Author
Schulze, S1, Author
Waldmann, Herbert2, Author           
Affiliations:
1external, ou_persistent22              
2Abt. IV: Chemische Biologie, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753290              

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Free keywords: SOLID-PHASE SYNTHESIS; PROTEIN-PHOSPHORYLATION; PHOSPHOTYROSYL PEPTIDE; SERINE; RAS; SIGNAL
 Abstract: Base-labile biologically relevant phosphopeptides can advantageously be synthesized under very mild conditions and without undesired side reactions by employing the heptyl (Hep) ester as C-terminal or the phenylacetamide (PhAc) as N-terminal enzyme-labile protecting group. The lipase-mediated saponification of the heptyl ester blocking function was successfully applied in the construction of a characteristic selectively phosphorylated hydroxyamino acid fragment of Raf-1 kinase, an important member of the so-called Ras signal transduction pathway.

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Language(s): eng - English
 Dates: 1997-09
 Publication Status: Issued
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: A1997YB90300020
 Degree: -

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Title: Synthesis
Source Genre: Journal
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Publ. Info: Stuttgart : Thieme Chemistry.
Pages: - Volume / Issue: 1997 (9) Sequence Number: - Start / End Page: 1098 - 1108 Identifier: ISSN: 0039-7881
CoNE: https://pure.mpg.de/cone/journals/resource/954925448757