ausblenden:
Schlagwörter:
SOLID-PHASE SYNTHESIS; PROTEIN-PHOSPHORYLATION; PHOSPHOTYROSYL PEPTIDE; SERINE; RAS; SIGNAL
Zusammenfassung:
Base-labile biologically relevant phosphopeptides can advantageously be synthesized under very mild conditions and without undesired side reactions by employing the heptyl (Hep) ester as C-terminal or the phenylacetamide (PhAc) as N-terminal enzyme-labile protecting group. The lipase-mediated saponification of the heptyl ester blocking function was successfully applied in the construction of a characteristic selectively phosphorylated hydroxyamino acid fragment of Raf-1 kinase, an important member of the so-called Ras signal transduction pathway.
