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  Helicobacter pylori outer membrane protein HopQ identified as a novel T4SS-associated virulence factor

Belogolova, E., Bauer, B., Pompaiah, M., Asakura, H., Brinkman, V., Ertl, C., et al. (2013). Helicobacter pylori outer membrane protein HopQ identified as a novel T4SS-associated virulence factor. Cellular Microbiology, 15(1), 1896-1912. doi:10.1111/cmi.12158.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-6925-B Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-8BF4-A
Genre: Journal Article

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http://onlinelibrary.wiley.com/wol1/doi/10.1111/cmi.12158/abstract (Publisher version)
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 Creators:
Belogolova, Elena, Author
Bauer, Bianca, Author
Pompaiah, Malvika, Author
Asakura, Hiroshi, Author
Brinkman, Volker, Author
Ertl, Claudia, Author
Bartfeld, Sina, Author
Nechitaylo, Taras Y.1, Author           
Haas, Rainer, Author
Machuy, Nikolaus, Author
Salama, Nina, Author
Churin, Yuri, Author
Meyer, Thomas F., Author
Affiliations:
1Max Planck Research Group Insect Symbiosis, MPI for Chemical Ecology, Max Planck Society, ou_421897              

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 Abstract: Helicobacter pylori is a bacterial pathogen that colonizes the gastric niche of ∼ 50% of the human population worldwide and is known to cause peptic ulceration and gastric cancer. Pathology of infection strongly depends on a cag pathogenicity island (cagPAI)-encoded type IV secretion system (T4SS). Here, we aimed to identify as yet unknown bacterial factors involved in cagPAI effector function and performed a large-scale screen of an H. pylori transposon mutant library using activation of the pro-inflammatory transcription factor NF-κB in human gastric epithelial cells as a measure of T4SS function. Analysis of ∼ 3000 H. pylori mutants revealed three non-cagPAI genes that affected NF-κB nuclear translocation. Of these, the outer membrane protein HopQ from H. pylori strain P12 was essential for CagA translocation and for CagA-mediated host cellresponses such as formation of the hummingbird phenotype and cell scattering. Besides that, deletion of hopQ reduced T4SS-dependent activation of NF-κB, induction of MAPK signalling and secretion of interleukin 8 (IL-8) in the host cells, but did not affect motility or the quantity of bacteria attached to host cells. Hence, we identified HopQ as a non-cagPAI-encoded cofactor of T4SS function.

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 Dates: 20132013-07-052013-11
 Publication Status: Issued
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 Identifiers: Other: KAL041
DOI: 10.1111/cmi.12158
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Title: Cellular Microbiology
Source Genre: Journal
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Publ. Info: Malden, MA : Blackwell Science
Pages: - Volume / Issue: 15 (1) Sequence Number: - Start / End Page: 1896 - 1912 Identifier: ISSN: 1462-5814
CoNE: https://pure.mpg.de/cone/journals/resource/959328105032