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  Interaction between AP-5 and the hereditary spastic paraplegia proteins SPG11 and SPG15

Hirst, J., Borner, G. H. H., Edgar, J., Hein, M. Y., Mann, M., Buchholz, F., et al. (2013). Interaction between AP-5 and the hereditary spastic paraplegia proteins SPG11 and SPG15. MOLECULAR BIOLOGY OF THE CELL, 24(16), 2558-2569. doi:10.1091/mbc.E13-03-0170.

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 Urheber:
Hirst, Jennifer1, Autor
Borner, Georg H. H.1, Autor
Edgar, James1, Autor
Hein, Marco Y.2, Autor           
Mann, Matthias2, Autor           
Buchholz, Frank1, Autor
Antrobus, Robin1, Autor
Robinson, Margaret S.1, Autor
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Schlagwörter: MOLECULAR ARCHITECTURE; STRUCTURE PREDICTION; COMPLEXES; QUANTIFICATION; IDENTIFICATION; SPATACSIN; SIGNALS; SERVER; GENE; AP2
 Zusammenfassung: The AP-5 complex is a recently identified but evolutionarily ancient member of the family of heterotetrameric adaptor proteins (AP complexes). It is associated with two proteins that are mutated in patients with hereditary spastic paraplegia, SPG11 and SPG15. Here we show that the four AP-5 subunits can be coimmunoprecipitated with SPG11 and SPG15, both from cytosol and from detergent-extracted membranes, with a stoichiometry of similar to 1:1:1:1:1:1. Knockdowns of SPG11 or SPG15 phenocopy knockdowns of AP-5 subunits:all six knockdowns cause the cation-independent mannose 6-phosphate receptor to become trapped in clusters of early endosomes. In addition, AP-5, SPG11, and SPG15 colocalize on a late endosomal/lysosomal compartment. Both SPG11 and SPG15 have predicted secondary structures containing alpha-solenoids related to those of clathrin heavy chain and COPI subunits. SPG11 also has an N-terminal, beta-propeller-like domain, which interacts in vitro with AP-5. We propose that AP-5, SPG15, and SPG11 form a coat-like complex, with AP-5 involved in protein sorting, SPG15 facilitating the docking of the coat onto membranes by interacting with PI3P via its FYVE domain, and SPG11 (possibly together with SPG15) forming a scaffold.

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Sprache(n): eng - English
 Datum: 2013
 Publikationsstatus: Erschienen
 Seiten: 12
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000324492900007
DOI: 10.1091/mbc.E13-03-0170
 Art des Abschluß: -

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Titel: MOLECULAR BIOLOGY OF THE CELL
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA : AMER SOC CELL BIOLOGY
Seiten: - Band / Heft: 24 (16) Artikelnummer: - Start- / Endseite: 2558 - 2569 Identifikator: ISSN: 1059-1524