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  Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission

Pietsch, J., Ma, X., Wehland, M., Aleshcheva, G., Schwarzwaelder, A., Segerer, J., et al. (2013). Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission. BIOMATERIALS, 34(31), 7694-7705. doi:10.1016/j.biomaterials.2013.06.054.

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Pietsch, Jessica1, Author
Ma, Xiao1, Author
Wehland, Markus1, Author
Aleshcheva, Ganna1, Author
Schwarzwaelder, Achim1, Author
Segerer, Jurgen1, Author
Birlem, Maria1, Author
Horn, Astrid1, Author
Bauer, Johann2, Author           
Infanger, Manfred1, Author
Grimm, Daniela1, Author
Affiliations:
1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: RANDOM POSITIONING MACHINE; HUMAN ENDOTHELIAL-CELLS; TISSUE GROWTH-FACTOR; SIMULATED MICROGRAVITY; CARCINOMA CELLS; EXTRACELLULAR-MATRIX; VASCULOGENIC MIMICRY; PARABOLIC FLIGHT; ALTERED GRAVITY; IN-VITROBiocompatibility; Bioreactor; Growth factors; Apoptosis; Polyetheretherketone; Thyroid cancer;
 Abstract: Human follicular thyroid cancer cells were cultured in Space to investigate the impact of microgravity on 3D growth. For this purpose, we designed and constructed a cell container that can endure enhanced physical forces, is connected to fluid storage chambers, performs media changes and cell harvesting automatically and supports cell viability. The container consists of a cell suspension chamber, two reserve tanks for medium and fixative and a pump for fluid exchange. The selected materials proved durable, non-cytotoxic, and did not inactivate RNAlater. This container was operated automatically during the unmanned Shenzhou-8 Space mission. FTC-133 human follicular thyroid cancer cells were cultured in Space for 10 days. Culture medium was exchanged after 5 days in Space and the cells were fixed after 10 days. The experiment revealed a scaffold-free formation of extraordinary large three-dimensional aggregates by thyroid cancer cells with altered expression of EGF and CTGF genes under real microgravity. (C) 2013 Elsevier Ltd. All rights reserved.

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Language(s): eng - English
 Dates: 2013-10
 Publication Status: Issued
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: BIOMATERIALS
Source Genre: Journal
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Publ. Info: THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND : ELSEVIER SCI LTD
Pages: - Volume / Issue: 34 (31) Sequence Number: - Start / End Page: 7694 - 7705 Identifier: ISSN: 0142-9612