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  Myosin motors fragment and compact membrane-bound actin filaments

Vogel, S. K., Petrasek, Z., Heinemann, F., & Schwille, P. (2013). Myosin motors fragment and compact membrane-bound actin filaments. eLife, 2: e00116. doi:10.7554/eLife.00116.001.

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 Creators:
Vogel, Sven K.1, Author           
Petrasek, Zdenek1, Author           
Heinemann, Fabian1, Author           
Schwille, Petra1, Author           
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1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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 Abstract: Cell cortex remodeling during cell division is a result of myofilament-driven contractility of the cortical membrane-bound actin meshwork. Little is known about the interaction between individual myofilaments and membrane-bound actin filaments. Here we reconstituted a minimal actin cortex to directly visualize the action of individual myofilaments on membrane-bound actin filaments using TIRF microscopy. We show that synthetic myofilaments fragment and compact membrane-bound actin while processively moving along actin filaments. We propose a mechanism by which tension builds up between the ends of myofilaments, resulting in compressive stress exerted to single actin filaments, causing their buckling and breakage. Modeling of this mechanism revealed that sufficient force (∼20 pN) can be generated by single myofilaments to buckle and break actin filaments. This mechanism of filament fragmentation and compaction may contribute to actin turnover and cortex reorganization during cytokinesis.

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Language(s): eng - English
 Dates: 2013-01-08
 Publication Status: Published online
 Pages: 18
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 Table of Contents: -
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 Identifiers: DOI: 10.7554/eLife.00116.001
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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 2 Sequence Number: e00116 Start / End Page: - Identifier: Other: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X