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  Cryo-electron tomography: The challenge of doing structural biology in situ

Lucic, V., Rigort, A., & Baumeister, W. (2013). Cryo-electron tomography: The challenge of doing structural biology in situ. JOURNAL OF CELL BIOLOGY, 202(3), 407-419. doi:10.1083/jcb.201304193.

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 Urheber:
Lucic, Vladan1, Autor           
Rigort, Alexander1, Autor           
Baumeister, Wolfgang1, Autor           
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Schlagwörter: TRANSMISSION ELECTRON-MICROSCOPY; DYNEIN REGULATORY COMPLEX; CELL BIOLOGY; CHLAMYDOMONAS-FLAGELLA; VITREOUS SECTIONS; LIGHT-MICROSCOPY; 3-DIMENSIONAL STRUCTURE; MOLECULAR ARCHITECTURE; CHEMORECEPTOR ARRAYS; DIRECT VISUALIZATION
 Zusammenfassung: Electron microscopy played a key role in establishing cell biology as a discipline, by producing fundamental insights into cellular organization and ultrastructure. Many seminal discoveries were made possible by the development of new sample preparation methods and imaging modalities. Recent technical advances include sample vitrification that faithfully preserves molecular structures, three-dimensional imaging by electron tomography, and improved image-processing methods. These new techniques have enabled the extraction of high fidelity structural information and are beginning to reveal the macromolecular organization of unperturbed cellular environments.

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Sprache(n): eng - English
 Datum: 2013
 Publikationsstatus: Erschienen
 Seiten: 13
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000322769400004
DOI: 10.1083/jcb.201304193
 Art des Abschluß: -

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Titel: JOURNAL OF CELL BIOLOGY
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA : ROCKEFELLER UNIV PRESS
Seiten: - Band / Heft: 202 (3) Artikelnummer: - Start- / Endseite: 407 - 419 Identifikator: ISSN: 0021-9525