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  alpha-Synuclein Oligomers with Broken Helical Conformation Form Lipoprotein Nanoparticles

Varkey, J., Mizuno, N., Hegde, B. G., Cheng, N., Steven, A. C., & Langen, R. (2013). alpha-Synuclein Oligomers with Broken Helical Conformation Form Lipoprotein Nanoparticles. JOURNAL OF BIOLOGICAL CHEMISTRY, 288(24), 17620-17630. doi:10.1074/jbc.M113.476697.

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 Creators:
Varkey, Jobin1, Author
Mizuno, Naoko2, Author           
Hegde, Balachandra G.1, Author
Cheng, Naiqian1, Author
Steven, Alasdair C.1, Author
Langen, Ralf1, Author
Affiliations:
1external, ou_persistent22              
2Mizuno, Naoko / Cellular and Membrane Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_1688137              

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Free keywords: HIGH-DENSITY-LIPOPROTEINS; APOLIPOPROTEIN-A-I; FAMILIAL PARKINSONS-DISEASE; PULSED ESR MEASUREMENTS; FATTY-ACIDS; TRANSGENIC MICE; MEMBRANE INTERACTIONS; UNILAMELLAR VESICLES; HIPPOCAMPAL-NEURONS; SECONDARY STRUCTURE
 Abstract: alpha-Synuclein (alpha S) is a membrane-binding protein with sequence similarity to apolipoproteins and other lipid-carrying proteins, which are capable of forming lipid-containing nanoparticles, sometimes referred to as "discs." Previously, it has been unclear whether alpha S also possesses this property. Using cryo-electron microscopy and light scattering, we found that alpha S can remodel phosphatidylglycerol vesicles into nanoparticles whose shape (ellipsoidal) and dimensions (in the 7-10-nm range) resemble those formed by apolipoproteins. The molar ratio of alpha S to lipid in nanoparticles is similar to 1:20, and alpha S is oligomeric (including trimers and tetramers). Similar nanoparticles form when alpha S is added to vesicles of mitochondrial lipids. This observation suggests a mechanism for the previously reported disruption of mitochondrial membranes by alpha S. Circular dichroism and four-pulse double electron electron resonance experiments revealed that in nanoparticles alpha S assumes a broken helical conformation distinct from the extended helical conformation adopted when alpha S is bound to intact vesicles or membrane tubules. We also observed alpha S-dependent tubule and nanoparticle formation in the presence of oleic acid, implying that alpha S can interact with fatty acids and lipids in a similar manner. alpha S-related nanoparticles might play a role in lipid and fatty acid transport functions previously attributed to this protein.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Issued
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000320380600053
DOI: 10.1074/jbc.M113.476697
 Degree: -

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Title: JOURNAL OF BIOLOGICAL CHEMISTRY
Source Genre: Journal
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Publ. Info: 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA : AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Pages: - Volume / Issue: 288 (24) Sequence Number: - Start / End Page: 17620 - 17630 Identifier: ISSN: 0021-9258