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  Role of Rck-Pat1b binding in assembly of processing-bodies

Ozgur, S., & Stoecklin, G. (2013). Role of Rck-Pat1b binding in assembly of processing-bodies. RNA BIOLOGY, 10(4), 528-539. doi:10.4161/rna.24086.

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 Urheber:
Ozgur, Sevim1, Autor           
Stoecklin, Georg2, Autor
Affiliations:
1Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              
2external, ou_persistent22              

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Schlagwörter: MESSENGER-RNA DECAY; DEAD BOX HELICASE; TRANSLATIONAL REPRESSION; SACCHAROMYCES-CEREVISIAE; P-BODIES; PAT1; DEADENYLATION; DEGRADATION; DHH1P; PROTEINSPatL1; Pat1b; RNA helicase; RNA-induced silencing complex; Rck; p54; decapping; mRNA degradation; processing body; translation suppression;
 Zusammenfassung: The DEAD box RNA helicase Rck and the scaffold protein Pat1b participate in controlling gene expression at the post-transcriptional level by suppressing mRNA translation and promoting mRNA decapping. In addition, both proteins are required for the assembly of processing (P)-bodies, cytoplasmic foci that contain stalled mRNAs and numerous components of the mRNA decay machinery. The C-terminal RecA-like domain of Rck interacts with the N-terminal acidic domain of Pat1b. Here, we identified point mutations in human Rck and Pat1b that prevent the two proteins from binding to each other. By analyzing interaction-deficient mutants in combination with knockdown and rescue strategies in human HeLa cells, we found that Pat1b assembles P-bodies and suppresses expression of tethered mRNAs in the absence of Rck binding. In contrast, Rck requires the Pat1b-binding site in order to promote P-body assembly and associate with the decapping enzyme Dcp2 as well as Ago2 and TNRC6A, two core components of the RNA-induced silencing complex. Our data indicate that P-body assembly occurs in a step-wise manner, where Rck participates in the initial suppression of mRNA translation, whereas Pat1b in a second step triggers P-body assembly and promotes mRNA decapping.

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Sprache(n): eng - English
 Datum: 2013
 Publikationsstatus: Erschienen
 Seiten: 12
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000321701600008
DOI: 10.4161/rna.24086
 Art des Abschluß: -

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Titel: RNA BIOLOGY
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA : LANDES BIOSCIENCE
Seiten: - Band / Heft: 10 (4) Artikelnummer: - Start- / Endseite: 528 - 539 Identifikator: ISSN: 1547-6286