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  Establishment of a robust single axis of cell polarity by coupling multiple positive feedback loops

Freisinger, T., Kluender, B., Johnson, J., Müller, N., Pichler, G., Beck, G., et al. (2013). Establishment of a robust single axis of cell polarity by coupling multiple positive feedback loops. NATURE COMMUNICATIONS, 4: 1807. doi:10.1038/ncomms2795.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-4641-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-4646-0
Genre: Journal Article

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 Creators:
Freisinger, Tina1, Author              
Kluender, Ben2, Author
Johnson, Jared2, Author
Müller, Nikola1, Author              
Pichler, Garwin1, Author              
Beck, Gisela1, Author              
Costanzo, Michael2, Author
Boone, Charles2, Author
Cerione, Richard A.2, Author
Frey, Erwin2, Author
Wedlich-Söldner, Roland1, Author              
Affiliations:
1Wedlich-Söldner, Roland / Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, Max Planck Society, escidoc:1565174              
2external, escidoc:persistent22              

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Free keywords: NUCLEOTIDE EXCHANGE FACTOR; SACCHAROMYCES-CEREVISIAE; SYMMETRY-BREAKING; CDC42 LOCALIZATION; MEMBRANE-PROTEINS; BUDDING YEAST; FACTOR CDC24P; RHO GDI; POLARIZATION; GTPASE
 Abstract: Establishment of cell polarity-or symmetry breaking-relies on local accumulation of polarity regulators. Although simple positive feedback is sufficient to drive symmetry breaking, it is highly sensitive to stochastic fluctuations typical for living cells. Here, by integrating mathematical modelling with quantitative experimental validations, we show that in the yeast Saccharomyces cerevisiae a combination of actin- and guanine nucleotide dissociation inhibitor-dependent recycling of the central polarity regulator Cdc42 is needed to establish robust cell polarity at a single site during yeast budding. The guanine nucleotide dissociation inhibitor pathway consistently generates a single-polarization site, but requires Cdc42 to cycle rapidly between its active and inactive form, and is therefore sensitive to perturbations of the GTPase cycle. Conversely, actin-mediated recycling of Cdc42 induces robust symmetry breaking but cannot restrict polarization to a single site. Our results demonstrate how cells optimize symmetry breaking through coupling between multiple feedback loops.

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Language(s): eng - English
 Dates: 2013-05
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000320589900003
DOI: 10.1038/ncomms2795
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Title: NATURE COMMUNICATIONS
Source Genre: Journal
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Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 4 Sequence Number: 1807 Start / End Page: - Identifier: ISSN: 2041-1723