Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes

Gibon, Y., Usadel, B., Blaesing, O. E., Kamlage, B., Hoehne, M., Trethewey, R., et al. (2006). Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes. Genome Biology, 7(8), R76. doi:10.1186/gb-2006-7-8-R76.

Item is

Dateien

einblenden: Dateien
ausblenden: Dateien
:
Gibon-2006-Integration of metab.pdf (beliebiger Volltext), 917KB
Name:
Gibon-2006-Integration of metab.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Gibon, Y.1, Autor           
Usadel, B.1, Autor           
Blaesing, O. E.2, Autor
Kamlage, B.2, Autor
Hoehne, M.1, Autor           
Trethewey, R.3, Autor           
Stitt, M.1, Autor           
Affiliations:
1System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
2External Organizations, ou_persistent22              
3Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              

Inhalt

einblenden:
ausblenden:
Schlagwörter: Amino Acids/metabolism Arabidopsis/*enzymology/genetics Carbon/metabolism Circadian Rhythm/*physiology Energy Metabolism/*physiology Gas Chromatography-Mass Spectrometry Gene Expression Profiling Gene Expression Regulation, Enzymologic/*physiology Genome, Plant/genetics/*physiology Mutation/genetics Nitrogen/metabolism Phosphoglucomutase/genetics RNA, Messenger/*metabolism
 Zusammenfassung: ABSTRACT: BACKGROUND: Genome-wide transcript profiling and analyses of enzyme activities from central carbon and nitrogen metabolism has shown that transcript levels undergo marked and rapid changes during diurnal cycles and after transfer to darkness, whereas changes of enzyme activities are smaller and delayed. In the starchless pgm mutant, where sugars are depleted every night, there are accentuated diurnal changes of transcript levels. Enzyme activities do not show larger diurnal changes; instead they shift towards the levels found in wild-type after several days of darkness. These results indicate that enzyme activities change slowly, integrating the changes of transcript levels over several diurnal cycles. RESULTS: To generalize this conclusion, 137 metabolites were profiled using GC-MS and LC-MS. Amplitudes of the diurnal changes of metabolites in pgm were (with the exception of sugars) similar or smaller than in wild-type. The average levels shifted towards those found after several days of darkness in wild-type. Examples include increased levels of many amino acids due to protein degradation, decreased levels of many fatty acids, increased tocopherol and decreased myo-inositol. Many metabolite-transcript correlations were found and the proportion of transcripts correlated with sugars increased dramatically in the starchless pgm mutant. CONCLUSION: Rapid diurnal changes of transcripts are integrated over time to generate quasi-stable changes across large sectors of metabolism. The slow response of enzyme activities and metabolites implies that correlations between metabolites and transcripts are due to regulation of gene expression by metabolites, rather than metabolites being changed as a consequence of a change in gene expression.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2006-08-192006
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Genome Biology
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 7 (8) Artikelnummer: - Start- / Endseite: R76 Identifikator: -