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  Combined transcript and metabolite profiling of Arabidopsis leaves reveals fundamental effects of the thiol-disulfide status on plant metabolism

Kolbe, A., Oliver, S. N., Fernie, A. R., Stitt, M., van Dongen, J. T., & Geigenberger, P. (2006). Combined transcript and metabolite profiling of Arabidopsis leaves reveals fundamental effects of the thiol-disulfide status on plant metabolism. Plant Physiology, 141(2), 412-422. doi:10.1104/pp.106.081208.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2A33-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2A34-7
Genre: Journal Article

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Kolbe-2006-Combined transcript.pdf (Any fulltext), 309KB
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 Creators:
Kolbe, A.1, Author
Oliver, S. N.2, Author              
Fernie, A. R.3, Author              
Stitt, M.4, Author              
van Dongen, J. T.2, Author              
Geigenberger, P.2, Author              
Affiliations:
1External Organizations, escidoc:persistent22              
2Storage Carbohydrate Metabolism, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, escidoc:1753336              
3Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, escidoc:1753339              
4System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, escidoc:1753327              

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Free keywords: adp-glucose pyrophosphorylase posttranslational redox-modification mitochondrial electron-transport amino-acid biosynthesis potato-tubers nitrate assimilation gene-expression thioredoxin targets starch synthesis root plastids
 Abstract: In this study, we used gas chromatography-mass spectrometry analysis in combination with flux analysis and the Affymetrix ATH1 GeneChip to survey the metabolome and transcriptome of Arabidopsis ( Arabidopsis thaliana) leaves in response to manipulation of the thiol-disulfide status. Feeding low concentrations of the sulfhydryl reagent dithiothreitol for 1 h at the end of the dark period led to posttranslational redox activation of ADP-glucose pyrophosphorylase and major alterations in leaf carbon partitioning, including an increased flux into major respiratory pathways, starch, cell wall, and amino acid synthesis, and a reduced flux to sucrose. This was accompanied by a decrease in the levels of hexose phosphates, while metabolites in the second half of the tricarboxylic acid cycle and various amino acids increased, indicating a stimulation of anaplerotic fluxes reliant on alpha-ketoglutarate. There was also an increase in shikimate as a precursor of secondary plant products and marked changes in the levels of the minor sugars involved in ascorbate synthesis and cell wall metabolism. Transcript profiling revealed a relatively small number of changes in the levels of transcripts coding for components of redox regulation, transport processes, and cell wall, protein, and amino acid metabolism, while there were no major alterations in transcript levels coding for enzymes involved in central metabolic pathways. These results provide a global picture of the effect of redox and reveal the utility of transcript and metabolite profiling as systemic strategies to uncover the occurrence of redox modulation in vivo.

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Language(s): eng - English
 Dates: 2006-05-022006
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000238168800017
DOI: 10.1104/pp.106.081208
ISSN: 0032-0889 (Print) 0032-0889 (Linking)
URI: ://000238168800017 http://www.plantphysiol.org/content/141/2/412.full.pdf
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Title: Plant Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 141 (2) Sequence Number: - Start / End Page: 412 - 422 Identifier: -