de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

 
 
 
 
DownloadE-Mail
  Combined transcript and metabolite profiling of Arabidopsis leaves reveals fundamental effects of the thiol-disulfide status on plant metabolism

Kolbe, A., Oliver, S. N., Fernie, A. R., Stitt, M., van Dongen, J. T., & Geigenberger, P. (2006). Combined transcript and metabolite profiling of Arabidopsis leaves reveals fundamental effects of the thiol-disulfide status on plant metabolism. Plant Physiology, 141(2), 412-422. doi:10.1104/pp.106.081208.

Item is

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2A33-9 Versions-Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2A34-7
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
Kolbe-2006-Combined transcript.pdf (beliebiger Volltext), 309KB
Beschreibung:
-
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Kolbe, A.1, Autor
Oliver, S. N.2, Autor              
Fernie, A. R.3, Autor              
Stitt, M.4, Autor              
van Dongen, J. T.2, Autor              
Geigenberger, P.2, Autor              
Affiliations:
1External Organizations, escidoc:persistent22              
2Storage Carbohydrate Metabolism, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, escidoc:1753336              
3Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, escidoc:1753339              
4System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, escidoc:1753327              

Inhalt

einblenden:
ausblenden:
Schlagwörter: adp-glucose pyrophosphorylase posttranslational redox-modification mitochondrial electron-transport amino-acid biosynthesis potato-tubers nitrate assimilation gene-expression thioredoxin targets starch synthesis root plastids
 Zusammenfassung: In this study, we used gas chromatography-mass spectrometry analysis in combination with flux analysis and the Affymetrix ATH1 GeneChip to survey the metabolome and transcriptome of Arabidopsis ( Arabidopsis thaliana) leaves in response to manipulation of the thiol-disulfide status. Feeding low concentrations of the sulfhydryl reagent dithiothreitol for 1 h at the end of the dark period led to posttranslational redox activation of ADP-glucose pyrophosphorylase and major alterations in leaf carbon partitioning, including an increased flux into major respiratory pathways, starch, cell wall, and amino acid synthesis, and a reduced flux to sucrose. This was accompanied by a decrease in the levels of hexose phosphates, while metabolites in the second half of the tricarboxylic acid cycle and various amino acids increased, indicating a stimulation of anaplerotic fluxes reliant on alpha-ketoglutarate. There was also an increase in shikimate as a precursor of secondary plant products and marked changes in the levels of the minor sugars involved in ascorbate synthesis and cell wall metabolism. Transcript profiling revealed a relatively small number of changes in the levels of transcripts coding for components of redox regulation, transport processes, and cell wall, protein, and amino acid metabolism, while there were no major alterations in transcript levels coding for enzymes involved in central metabolic pathways. These results provide a global picture of the effect of redox and reveal the utility of transcript and metabolite profiling as systemic strategies to uncover the occurrence of redox modulation in vivo.

Details

einblenden:
ausblenden:
Sprache(n): eng - Englisch
 Datum: 2006-05-022006
 Publikationsstatus: Im Druck publiziert
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: ISI:000238168800017
DOI: 10.1104/pp.106.081208
ISSN: 0032-0889 (Print) 0032-0889 (Linking)
URI: ://000238168800017 http://www.plantphysiol.org/content/141/2/412.full.pdf
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Plant Physiology
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 141 (2) Artikelnummer: - Start- / Endseite: 412 - 422 Identifikator: -