ausblenden:
Schlagwörter:
Arabidopsis/enzymology
Chloroplasts/enzymology/*genetics/metabolism
DNA-Directed RNA Polymerases/metabolism
Genes, Bacterial
*Genes, Mitochondrial
*Genes, Plant
Genome, Plastid
Mitochondria/enzymology
Oenothera/genetics
Plants, Genetically Modified/genetics/metabolism
*Promoter Regions (Genetics)
Tobacco/genetics
Transcription Initiation Site
*Transcription, Genetic
Transgenes
Zusammenfassung:
The transcriptional machineries of plastids and mitochondria in higher plants exhibit striking similarities. All mitochondrial genes and part of the plastid genes are transcribed by related phage-type RNA polymerases. Furthermore, the majority of mitochondrial promoters and a subset of plastid promoters show a similar structural organization. We show here that the plant mitochondrial atpA promoter is recognized by plastid RNA polymerases in vitro and in vivo. The Arabidopsis phage-type RNA polymerase RpoTp, an enzyme localized exclusively to plastids, was found to recognize the mitochondrial atpA promoter in in vitro assays suggesting the possibility that mitochondrial promoters might function as well in plastids. We have, therefore, generated transplastomic tobacco plants harboring in their chloroplast genome the atpA promoter fused to the coding region of the bacterial nptII gene. The chimeric nptII gene was found to be efficiently transcribed in chloroplasts. Mapping of the 5' ends of the nptII transcripts revealed accurate recognition of the atpA promoter by the chloroplast transcription machinery. We show further that the 5' untranslated region (UTR) of the mitochondrial atpA transcript is capable of mediating translation in chloroplasts. The functional and evolutionary implications of these findings as well as possible applications in chloroplast genome engineering are discussed.
