de.mpg.escidoc.pubman.appbase.FacesBean
English
 
Help Guide Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Protection of liposomes against fusion during drying by oligosaccharides is not predicted by the calorimetric glass transition temperatures of the dry sugars

Hincha, D. K., Rennecke, P., & Oliver, A. E. (2008). Protection of liposomes against fusion during drying by oligosaccharides is not predicted by the calorimetric glass transition temperatures of the dry sugars. European Biophysics Journal with Biophysics Letters, 37(4), 503-508. doi:10.1007/s00249-007-0245-0.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-277B-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0018-CF76-E
Genre: Journal Article

Files

show Files
hide Files
:
Hincha-2008-Protection of liposo.pdf (Any fulltext), 433KB
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Hincha, D. K.1, Author              
Rennecke, P.2, Author
Oliver, A. E.2, Author
Affiliations:
1Transcript Profiling, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, escidoc:1753306              
2External Organizations, escidoc:persistent22              

Content

show
hide
Free keywords: model membranes d-sorbitol stability water phosphatidylcholine anhydrobiotes stabilization vitrification preservation tolerance
 Abstract: Sugars play an important role in the desiccation tolerance of most anhydrobiotic organisms. It has been shown in previous studies that different structural families of oligosaccharides have different efficacies to interact with phospholipid headgroups and protect membranes from solute leakage during drying. Here, we have compared three families of linear oligosaccharides (fructans (inulins), malto-oligosaccharides, manno-oligosaccharides) for their chain-length dependent protection of egg phosphatidylcholine liposomes against membrane fusion. We found increased protection with chain length up to a degree of polymerization (DP) of 5 for malto-oligosaccharides, and a decrease for inulins and manno-oligosaccharides. Differential scanning calorimetry measurements showed that for all sugars the glass transition temperature (T-g) increased with DP, although to different degrees for the different oligosaccharide families. Higher T-g values resulted in reduced membrane fusion only for malto-oligosaccharides below DP5. Contrary to expectation, for inulins, manno-oligosaccharides and malto-oligosaccharides of a DP above five, fusion increased with increasing T-g, indicating that other physical parameters are more important in determining the ability of different sugars to protect membranes against fusion during drying. Further research will be necessary to experimentally define such parameters.

Details

show
hide
Language(s): eng - English
 Dates: 2007-12-082008
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: ISI:000254235700015
DOI: 10.1007/s00249-007-0245-0
ISSN: 0175-7571 (Print)0175-7571 (Linking)
URI: ://000254235700015http://www.springerlink.com/content/k16q6k37q670675m/fulltext.pdf
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: European Biophysics Journal with Biophysics Letters
  Other : European Biophysics Journal with Biophysics Letters
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Berlin : Springer
Pages: - Volume / Issue: 37 (4) Sequence Number: - Start / End Page: 503 - 508 Identifier: ISSN: 0175-7571
CoNE: http://pubman.mpdl.mpg.de/cone/journals/resource/954925487773