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  Identification of a xylogalacturonan xylosyltransferase involved in pectin biosynthesis in Arabidopsis

Jensen, J. K., Sorensen, S. O., Harholt, J., Geshi, N., Sakuragi, Y., Moller, I., et al. (2008). Identification of a xylogalacturonan xylosyltransferase involved in pectin biosynthesis in Arabidopsis. Plant Cell, 20(5), 1289-1302. doi:10.1105/tpc.107.050906.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-2767-F Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-2768-D
Genre: Journal Article

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 Creators:
Jensen, J. K.1, Author
Sorensen, S. O.1, Author
Harholt, J.1, Author
Geshi, N.1, Author
Sakuragi, Y.1, Author
Moller, I.1, Author
Zandleven, J.1, Author
Bernal, A. J.1, Author
Jensen, N. B.1, Author
Sorensen, C.1, Author
Pauly, M.1, Author
Beldman, G.1, Author
Willats, W. G. T.1, Author
Scheller, H. V.1, Author
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1External Organizations, ou_persistent22              

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Free keywords: polysaccharide rhamnogalacturonan-ii cell-wall polysaccharides monoclonal-antibody xyloglucan galactosyltransferase enzymatic degradation rapid method gene homogalacturonan thaliana encodes
 Abstract: Xylogalacturonan (XGA) is a class of pectic polysaccharide found in plant cell walls. The Arabidopsis thaliana locus At5g33290 encodes a predicted Type II membrane protein, and insertion mutants of the At5g33290 locus had decreased cell wall xylose. Immunological studies, enzymatic extraction of polysaccharides, monosaccharide linkage analysis, and oligosaccharide mass profiling were employed to identify the affected cell wall polymer. Pectic XGA was reduced to much lower levels in mutant than in wild-type leaves, indicating a role of At5g33290 in XGA biosynthesis. The mutated gene was designated xylogalacturonan deficient1 (xgd1). Transformation of the xgd1-1 mutant with the wild-type gene restored XGA to wild-type levels. XGD1 protein heterologously expressed in Nicotiana benthamiana catalyzed the transfer of xylose from UDP-xylose onto oligogalacturonides and endogenous acceptors. The products formed could be hydrolyzed with an XGA-specific hydrolase. These results confirm that the XGD1 protein is a XGA xylosyltransferase. The protein was shown by expression of a fluorescent fusion protein in N. benthamiana to be localized in the Golgi vesicles as expected for a glycosyltransferase involved in pectin biosynthesis.

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Language(s): eng - English
 Dates: 2008-05-082008
 Publication Status: Issued
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 Identifiers: ISI: ISI:000257320600010
DOI: 10.1105/tpc.107.050906
ISSN: 1040-4651 (Print) 1040-4651 (Linking)
URI: ://000257320600010 http://www.plantcell.org/content/20/5/1289.full.pdf
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Title: Plant Cell
Source Genre: Journal
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Pages: - Volume / Issue: 20 (5) Sequence Number: - Start / End Page: 1289 - 1302 Identifier: -