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  Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli

Neupert, J., Karcher, D., & Bock, R. (2008). Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research, 36(19), e124. doi:10.1093/nar/gkn545.

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Neupert, J.1, Author           
Karcher, D.1, Author           
Bock, R.1, Author           
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1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: thermosensor controls expression translation transformation riboswitches
 Abstract: RNA thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts. Here, we report the de novo design of considerably simpler RNA thermometers that provide useful RNA-only tools to regulate bacterial gene expression by a shift in the growth temperature. We show that a single small stem-loop structure containing the ribosome binding site is sufficient to construct synthetic RNA thermometers that work efficiently at physiological temperatures. Our data suggest that the thermometers function by a simple melting mechanism and thus provide minimum size on/off switches to experimentally induce or repress gene expression by temperature.

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Language(s): eng - English
 Dates: 2008-08-302008
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000260983000003
DOI: 10.1093/nar/gkn545
ISSN: 1362-4962 (Electronic) 0305-1048 (Linking)
URI: ://000260983000003 http://nar.oxfordjournals.org/content/36/19/e124 http://nar.oxfordjournals.org/content/36/19/e124.full.pdf
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 36 (19) Sequence Number: - Start / End Page: e124 Identifier: -