English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes

Bazzini, A. A., Manacorda, C. A., Tohge, T., Conti, G., Rodriguez, M. C., Nunes-Nesi, A., et al. (2011). Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes. PLoS One, 6(12), e28466. doi:10.1371/journal.pone.0028466.

Item is

Files

show Files
hide Files
:
Bazzini-2011-Metabolic and miRNA.pdf (Any fulltext), 2MB
Name:
Bazzini-2011-Metabolic and miRNA.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Bazzini, A. A.1, Author
Manacorda, C. A.1, Author
Tohge, T.2, Author           
Conti, G.1, Author
Rodriguez, M. C.1, Author
Nunes-Nesi, A.2, Author           
Villanueva, S.1, Author
Fernie, A. R.2, Author           
Carrari, F.2, Author           
Asurmendi, S.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

Content

show
hide
Free keywords: tobacco-mosaic-virus systemic acquired-resistance protein-mediated resistance rna silencing suppressors arabidopsis-thaliana gene-expression transgenic tobacco disease resistance gas-chromatography nicotiana-tabacum
 Abstract: Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration.

Details

show
hide
Language(s): eng - English
 Dates: 2011-12-122011
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000298366600019
DOI: 10.1371/journal.pone.0028466
ISSN: 1932-6203
URI: ://000298366600019http://www.plosone.org/article/fetchObjectAttachment.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0028466&representation=PDF
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS One
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 6 (12) Sequence Number: - Start / End Page: e28466 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850