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  Selection of Shine-Dalgarno sequences in plastids

Drechsel, O., & Bock, R. (2011). Selection of Shine-Dalgarno sequences in plastids. Nucleic Acids Research, 39(4), 1427-1438. doi:10.1093/nar/gkq978.

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Drechsel-2011-Selection of Shine-D.pdf (beliebiger Volltext), 6MB
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Drechsel, O.1, Autor           
Bock, R.2, Autor           
Affiliations:
1Integrative Carbon Biology, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753329              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Schlagwörter: organelle ribosome chloroplast messenger-rna gene-expression higher-plants chlamydomonas-reinhardtii 5'-untranslated region translation regulation transgene containment tobacco chloroplasts initiation complex
 Zusammenfassung: Like bacterial genes, most plastid (chloroplast) genes are arranged in operons and transcribed as polycistronic mRNAs. Plastid protein biosynthesis occurs on bacterial-type 70S ribosomes and translation initiation of many (but not all) mRNAs is mediated by Shine-Dalgarno (SD) sequences. To study the mechanisms of SD sequence recognition, we have analyzed translation initiation from mRNAs containing multiple SD sequences. Comparing translational efficiencies of identical transgenic mRNAs in Escherichia coli and plastids, we find surprising differences between the two systems. Most importantly, while internal SD sequences are efficiently recognized in E. coli, plastids exhibit a bias toward utilizing predominantly the 5'-most SD sequence. We propose that inefficient recognition of internal SD sequences provides the raison d'etre for most plastid polycistronic transcripts undergoing post-transcriptional cleavage into monocistronic mRNAs.

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Sprache(n): eng - English
 Datum: 2010-10-212011
 Publikationsstatus: Erschienen
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 Identifikatoren: ISI: ISI:000288019400030
DOI: 10.1093/nar/gkq978
ISSN: 0305-1048
URI: ://000288019400030 http://nar.oxfordjournals.org/content/39/4/1427.full.pdf
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Titel: Nucleic Acids Research
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 39 (4) Artikelnummer: - Start- / Endseite: 1427 - 1438 Identifikator: -