ausblenden:
Schlagwörter:
organelle ribosome chloroplast
messenger-rna
gene-expression
higher-plants
chlamydomonas-reinhardtii
5'-untranslated region
translation regulation
transgene containment
tobacco chloroplasts
initiation complex
Zusammenfassung:
Like bacterial genes, most plastid (chloroplast) genes are arranged in operons and transcribed as polycistronic mRNAs. Plastid protein biosynthesis occurs on bacterial-type 70S ribosomes and translation initiation of many (but not all) mRNAs is mediated by Shine-Dalgarno (SD) sequences. To study the mechanisms of SD sequence recognition, we have analyzed translation initiation from mRNAs containing multiple SD sequences. Comparing translational efficiencies of identical transgenic mRNAs in Escherichia coli and plastids, we find surprising differences between the two systems. Most importantly, while internal SD sequences are efficiently recognized in E. coli, plastids exhibit a bias toward utilizing predominantly the 5'-most SD sequence. We propose that inefficient recognition of internal SD sequences provides the raison d'etre for most plastid polycistronic transcripts undergoing post-transcriptional cleavage into monocistronic mRNAs.