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  Calcium-induced calcium release participates in cell volume regulation of rabbit TALH cells

Tinel, H., Kinne-Saffran, E., & Kinne, R. K. H. (2002). Calcium-induced calcium release participates in cell volume regulation of rabbit TALH cells. Pflügers Archiv - European Journal of Physiology, 443(5-6): 1, pp. 754-761. Retrieved from http://dx.doi.org/10.1007/s00424-001-0774-9.

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Tinel, Hanna1, Autor
Kinne-Saffran, Evamaria2, Autor           
Kinne, Rolf K. H.2, Autor           
Affiliations:
1Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753286              
2Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753294              

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Schlagwörter: calcium channel; calcium signalling; calcium stores; osmoregulation; regulatory volume decrease
 Zusammenfassung: Changes of cell volume and intracellular calcium concentration ([Ca2+](i)) in immortalized thick ascending limb of Henle's loop (TALH) cells were monitored using confocal laser scanning microscopy and fura-2 fluorescence, respectively, Reduction of the extracellular osmolarity from 600 to 300 mosmol/l induced cell swelling followed by regulatory volume decrease (RVD). Simultaneously, the [Ca2+](i) increased transiently. The calcium rise was not observed in calcium-free solution or in the presence of nifedipine, indicating that the change was, in the first place, due to the activation of a calcium influx. Application of ATP or caffeine in isotonic solutions increased transiently the [Ca2+](i), which revealed the existence of stores in TALH cells sensitive to inositol-1,4,5 trisphosphate (IP3) and ryanodine. To examine the possibility that the calcium influx might induce calcium release, manganese quenching experiments were performed. In hypotonic calcium-free solutions, the decay of the calcium-insensitive and calcium- sensitive fluorescence occurred simultaneously. In the presence of extracellular calcium however, the calcium-sensitive wavelength revealed initial calcium influx followed by a calcium release from intracellular stores. Thus, the calcium influx was a prerequisite for the calcium release. We conclude that calcium-induced calcium release participates in global calcium signalling during RVD of TALH cells.

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Sprache(n): eng - English
 Datum: 2002-02-05
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 6151
URI: http://dx.doi.org/10.1007/s00424-001-0774-9
 Art des Abschluß: -

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Titel: Pflügers Archiv - European Journal of Physiology
Genre der Quelle: Zeitschrift
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Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 443 (5-6) Artikelnummer: 1 Start- / Endseite: 754 - 761 Identifikator: -