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  Hepatocyte nuclear factor-4 (HNF-4α) is not involved in hypoxia induced Erythropoietin regulation in human neuroblastoma cell lines

Stolze, I., Berchner-Pfannschmidt, U., Freitag, P., Frede, S., & Fandrey, J. (2002). Hepatocyte nuclear factor-4 (HNF-4α) is not involved in hypoxia induced Erythropoietin regulation in human neuroblastoma cell lines.

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Genre: Conference Paper
Alternative Title : FASEB J.

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 Creators:
Stolze, Ineke, Author
Berchner-Pfannschmidt, Utta1, Author
Freitag, Patricia, Author
Frede, Stilla, Author
Fandrey, Joachim, Author
Affiliations:
1Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753286              

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 Abstract: Oxygen dependent Erythropoietin (EPO) production is regulated by hypoxia inducible factor-1 (HIF-1) which consists of the O2-labile α- and the constitutive β-subunit. In human hepatoma cells (HepG2) HNF-4α enhances hypoxia-induced EPO expression. Herein, we investigated EPO gene regulation in human neuroblastoma (NB) cell lines. Methods: EPO production in SH-SY5Y, Kelly and HepG2 cells was measured by real time PCR and EPO elisa. Intracellular distribution of HIF-1α, HIF-1β and HNF-4α was studied by Western blot and immunohistochemistry. Cells were transiently transfected with HIF-1-driven luciferase and a dominant negative (dn) HNF-4 plasmid. Results: Hypoxia induced EPO gene expression in NB cells was maximal at <0.1% O2. HIF-1α protein accumulated and translocated to the nucleus at an oxygen threshold of 7% O2 in HepG2 cells but at 3% O2 in NB. Differences were also seen in HIF-1α protein degradation upon reoxygenation. Very little HNF-4α was expressed in NB, but did not colocalize with HIF-1α under hypoxic conditions. Transfection with dnHNF-4 did not affect hypoxic induction of EPO in NB cells. Conclusions: HIF-1, but not HNF-4α is involved in EPO gene regulation in human NB cell lines. (This abstract is as it is in the printed issue of the journal).

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Language(s): eng - English
 Dates: 2002-03-20
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 17749
ISI: 000174533602500
 Degree: -

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Title: Experimental Biology 2002
Place of Event: New Orleans, Louisiana, USA
Start-/End Date: 2002-04-20 - 2002-04-24

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Title: Abstracts Part I
Source Genre: Issue
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Pages: - Volume / Issue: - Sequence Number: 386.3 Start / End Page: A451 - A452 Identifier: -

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Title: FASEB Journal
  Alternative Title : FASEB J.
Source Genre: Journal
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Pages: - Volume / Issue: 16 (4) Sequence Number: - Start / End Page: - Identifier: ISSN: 0892-6638