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  Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE

Buchwald, G., Friebel, A., Galán, J. E., Hardt, W.-D., Wittinghofer, A., & Scheffzek, K. (2002). Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE. EMBO Journal, 21(13): 1, pp. 3286-3295. Retrieved from http://emboj.oupjournals.org/cgi/content/abstract/21/13/3286?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&searchid=1052749461805_2739&stored_search=&FIRSTINDEX=0&volume=21&firstpage=3286&journalcode=emboj.

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Genre: Journal Article
Alternative Title : Embo J.

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 Creators:
Buchwald, Gretel1, Author
Friebel, Andrea, Author
Galán, Jorge E., Author
Hardt, Wolf-Dietrich, Author
Wittinghofer, Alfred2, Author           
Scheffzek, Klaus, Author
Affiliations:
1Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753286              
2Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753294              

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Free keywords: cytoskeleton; Dbl; GEF; GTP binding; Salmonella
 Abstract: The bacterial enteropathogen Salmonella typhimurium employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family GTP-binding protein-dependent signaling cascades to induce cytoskeletal rearrangements. One of these translocated Salmonella toxins, SopE, can activate Cdc42 in a Dbl-like fashion despite its lack of sequence similarity to Dbl-like proteins, the Rho-specific eukaryotic guanine nucleotide exchange factors. To elucidate the mechanism of SopE-mediated guanine nucleotide exchange, we have analyzed the structure of the complex between a catalytic fragment of SopE and Cdc42. SopE binds to and locks the switch I and switch II regions of Cdc42 in a conformation that promotes guanine nucleotide release. This conformation is strikingly similar to that of Rac1 in complex with the eukaryotic Dbl-like exchange factor Tiam1. However, the catalytic domain of SopE has an entirely different architecture from that of Tiam1 and interacts with the switch regions via different amino acids. Therefore, SopE represents the first example of a non-Dbl-like protein capable of inducing guanine nucleotide exchange in Rho family proteins.

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Language(s): eng - English
 Dates: 2002-07-01
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: EMBO Journal
  Alternative Title : Embo J.
Source Genre: Journal
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Pages: - Volume / Issue: 21 (13) Sequence Number: 1 Start / End Page: 3286 - 3295 Identifier: -