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  Mutational, structural and dynamic analysis of the Ran-RanBP2 interaction in nucleo-cytoplasmic transport

Zhao, X. (2002). Mutational, structural and dynamic analysis of the Ran-RanBP2 interaction in nucleo-cytoplasmic transport. PhD Thesis, Ruhr-Universität Bochum, Bochum.

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 Creators:
Zhao, Xiaodong1, Author
Wittinghofer, Alfred1, Referee           
Affiliations:
1Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society, Otto-Hahn-Str. 11, 44227 Dortmund, DE, ou_1753294              

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Free keywords: Transferreaktion; NMR-Spektroskopie; Guanosintriphosphat; Kinetik; Chemische Bindung
 Abstract: The functions of some conserved motifs of Ran and Ran binding domain 1 and 2 of RanBP2 were characterized by the kinetics and equilibrium of spectroscopy using analogues of guanine nucleotides, Isothermal Titration Calorimetry (ITC) and structural analysis (crystallization and NMR) approaches. The results suggest 1) The C-terminal DEDDDL motif destabilizes GTP complexation with Ran. This tail of Ran is important for binding to BD1. 2) The N-terminal fragment of BD1 contributes largely to the recognition of Ran in its GTP form. 3) The conserved 57WKER motif of BD1 stabilizes GTP binding on Ran by interactions with the effector loop of Ran. 4) BD1 binds to Ran in its GDP form with a weak affinity of 50 μM. It promotes the dissociation rate of GDP from the Ran*GDP complex putatively by deformation of α1b and α1a of Ran in the GDP form. 5) NMR studies reveal that there is an extra 5th -β strand in the core domain of BD2 in solution, which make BD2 a perfect canonical PH domain.

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Language(s): eng - English
 Dates: 2002-07-16
 Publication Status: Accepted / In Press
 Pages: 135 pp.
 Publishing info: Bochum : Ruhr-Universität Bochum
 Table of Contents: -
 Rev. Type: -
 Degree: PhD

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