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Schlagwörter:
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Zusammenfassung:
Phlorizin, a phloretin 2'-glucoside, is a potent inhibitor of the Na+/glucose cotransporter (SGLT1). On the basis of transport studies in intact cells, a binding
site for phlorizin was suggested in the region between amino acids 604-610 of the C-terminal loop 13. To further investigate phlorizin binding titration
experiments of the intrinsic Trp fluorescence of isolated wild-type loop 13 and two mutated loops (Y604K and G609K) were carried out. Phlorizin (135
M) produced approximately 40% quenching of the fluorescence of wild-type loop 13; quenching could also be observed with the two mutated loops. The
apparent Kd was lowest for the wild-type loop 13 (Kd 23 M), followed by mutant G609K (57 M) and mutant Y604K (70 M). Binding of phlorizin
was further confirmed by a decrease of the accessibility of loop 13 to the collisional quencher acrylamide. The interaction involves the aromatic moiety of
the aglucone since phloretin (the aglucone of phlorizin) showed almost the same effects as phlorizin, while D-glucose did not. MALDI-TOF experiments
revealed that loop 13 contained a disulfide bond between Cys 560 and Cys 608 that is very important for phlorizin-dependent fluorescence quenching.
These studies provide direct evidence that loop 13 is a site (important amino acids including 604-609) for the molecular interaction between SGLT1 and
phlorizin. They confirm that the aglucone part of the glucoside is responsible for this interaction.
