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  Structural fingerprints of the Ras-GTPase activating proteins neurofibromin and p120GAP

Ahmadian, M. R., Kiel, C., Stege, P., & Scheffzek, K. (2003). Structural fingerprints of the Ras-GTPase activating proteins neurofibromin and p120GAP. Journal of Molecular Biology, 329(4): 1, pp. 699-710. Retrieved from http://dx.doi.org/10.1016/S0022-2836(03)00514-X.

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Genre: Zeitschriftenartikel
Alternativer Titel : J. Mol. Biol.

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 Urheber:
Ahmadian, Mohammad Reza1, Autor           
Kiel, Christina2, Autor
Stege, Patricia2, Autor
Scheffzek, Klaus, Autor
Affiliations:
1Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753294              
2Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753286              

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Schlagwörter: Ras; GAP; p120GAP; NF1; neurofibromin TRANSITION-STATE ANALOG; GAP-RELATED DOMAIN; TYPE-1 NF1 GENE; SIGNAL-TRANSDUCTION; ARGININE-FINGER; ONCOGENIC RAS; TUMOR PREDISPOSITION; PLECKSTRIN HOMOLOGY; LEARNING-DEFICITS; ALUMINUM FLUORIDE
 Zusammenfassung: Ras specific GTPase activating proteins (GAPs), neurofibromin and p120GAP, bind GTP bound Ras and efficiently complement its active site. Here we present comparative data from mutations and fluorescence-based assays of the catalytic domains of both RasGAPs and interpret them using the crystal structures. Three prominent regions in RasGAPs, the arginine-finger loop, the phenylalanine-leucine-arginine (FLR) region and alpha7/variable loop contain structural fingerprints governing the GAP function. The finger loop is crucial for the stabilization of the transition state of the GTPase reaction. This function is controlled by residues proximal to the catalytic arginine, which are strikingly different between the two RasGAPs. These residues specifically determine the orientation and therefore the positioning of the arginine finger in the Ras active site. The invariant FLR region, a hallmark for RasGAPs, indirectly contributes to GTPase stimulation by forming a scaffold, which stabilizes Ras switch regions. We show that a long hydrophobic side-chain in the FLR region is crucial for this function. The alpha7/variable loop uses several conserved residues including two lysine residues, which are involved in numerous interactions with the switch I region of Ras. This region determines the specificity of the Ras-RasGAP interaction.

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Sprache(n): eng - English
 Datum: 2003-06-13
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 34951
URI: http://dx.doi.org/10.1016/S0022-2836(03)00514-X
 Art des Abschluß: -

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Titel: Journal of Molecular Biology
  Alternativer Titel : J. Mol. Biol.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 329 (4) Artikelnummer: 1 Start- / Endseite: 699 - 710 Identifikator: -