English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Highly Rapid Amplification-Free and Quantitative DNA Imaging Assay

Klamp, T., Camps, M., Nieto, B., Guasch, F., Ranasinghe, R. T., Wiedemann, J., et al. (2013). Highly Rapid Amplification-Free and Quantitative DNA Imaging Assay. SCIENTIFIC REPORTS, 3: 1852. doi:10.1038/srep01852.

Item is

Files

show Files
hide Files
:
srep01852.pdf (Any fulltext), 3MB
Name:
srep01852.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
open access article
License:
-

Locators

show

Creators

show
hide
 Creators:
Klamp, Tobias1, Author
Camps, Marta1, Author
Nieto, Benjamin1, Author
Guasch, Francesc1, Author
Ranasinghe, Rohan T.1, Author
Wiedemann, Jens2, Author           
Petrasek, Zdenek2, Author           
Schwille, Petra2, Author           
Klenerman, David1, Author
Sauer, Markus1, Author
Affiliations:
1external, ou_persistent22              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

Content

show
hide
Free keywords: NUCLEIC-ACIDS; GENOMIC DNA; FLOW-CYTOMETRY; HYBRIDIZATION; SEQUENCES; FLUORESCENCE; NANOPARTICLES; RNA
 Abstract: There is an urgent need for rapid and highly sensitive detection of pathogen-derived DNA in a point-of-care (POC) device for diagnostics in hospitals and clinics. This device needs to work in a 'sample-in-result-out' mode with minimum number of steps so that it can be completely integrated into a cheap and simple instrument. We have developed a method that directly detects unamplified DNA, and demonstrate its sensitivity on realistically sized 5 kbp target DNA fragments of Micrococcus luteus in small sample volumes of 20 mu L. The assay consists of capturing and accumulating of target DNA on magnetic beads with specific capture oligonucleotides, hybridization of complementary fluorescently labeled detection oligonucleotides, and fluorescence imaging on a miniaturized wide-field fluorescence microscope. Our simple method delivers results in less than 20 minutes with a limit of detection (LOD) of similar to 5 pM and a linear detection range spanning three orders of magnitude.

Details

show
hide
Language(s): eng - English
 Dates: 2013-05-16
 Publication Status: Published online
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000318965000002
DOI: 10.1038/srep01852
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: SCIENTIFIC REPORTS
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 3 Sequence Number: 1852 Start / End Page: - Identifier: ISSN: 2045-2322