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  Freeze-substitution of Drososphila heads for subsequent 3H-2-deoxyglucose autoradiography

Rodrigues, V., & Bülthoff, I. (1985). Freeze-substitution of Drososphila heads for subsequent 3H-2-deoxyglucose autoradiography. Journal of Neuroscience Methods, 13(3-4), 183-190. doi:10.1016/0165-0270(85)90066-4.

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Rodrigues, V, Author
Bülthoff, I1, Author           
Affiliations:
1Department Human Perception, Cognition and Action, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497797              

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 Abstract: High resolution of [3H]2-deoxyglucose labelling was obtained in autoradiographs of Drosophila brains after freeze-substitution in anhydrous acetone at −76°C. This method was applied to preparations which received visual, olfactory and mechanosensory stimulation. The autoradiographs were compared to those obtained after freeze-drying. Freeze-substitution, which has proved to be technically simple, rapid and inexpensive, yields a good quality of tissue preservation and hence is recommended for tissue dehydration prior to autoradiography.

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 Dates: 1985-05
 Publication Status: Issued
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Title: Journal of Neuroscience Methods
Source Genre: Journal
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Pages: - Volume / Issue: 13 (3-4) Sequence Number: - Start / End Page: 183 - 190 Identifier: -