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  Analysis of estrogenic compounds in Polygonum cuspidatum by bioassay and high performance liquid chromatography

Zhang, C., Zhang, X., Zhang Y, Xu Q, Xiao, H., & Liang, X. (2006). Analysis of estrogenic compounds in Polygonum cuspidatum by bioassay and high performance liquid chromatography. Journal of Ethnopharmacology, 105(1-2), 223-228. doi:10.1016/j.jep.2005.10.029.

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資料種別: 学術論文

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 作成者:
Zhang, C, 著者
Zhang, X1, 著者           
Zhang Y, Xu Q, Xiao, H, 著者
Liang, X, 著者
所属:
1Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497798              

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 要旨: The estrogenic activity of traditional Chinese herb-Polygonum cuspidatum Sieb. et Zucc. was investigated by a recombinant yeast screening (YES) assay. Anthraquinones are the main components in the plant, of which emodin is the most abundant one. The ethyl acetate fraction of the ethanol extract of Polygonum cuspidatum was separated on a silica gel TLC plate and seven sub-fractions were collected. The results of bioassay demonstrated that Hzs1 and Hzs6 showed higher estrogenic activities than that of others and the potency of these two compounds were approximately 10(-4) g/L and 10(-3) g/L, respectively. HPLC analysis was performed to determine the activities and the active components. Combining the results of HPLC analysis and estrogenic activity test by YES led to the conclusion that an unknown bioactive compound might exist in the extraction of Polygonum cuspidatum.

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 日付: 2006-04
 出版の状態: 出版
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出版物名: Journal of Ethnopharmacology
種別: 学術雑誌
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出版社, 出版地: -
ページ: - 巻号: 105 (1-2) 通巻号: - 開始・終了ページ: 223 - 228 識別子(ISBN, ISSN, DOIなど): -