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  Glutamine-free media for vaccine production processes

Genzel, Y., Alt, R., & Reichl, U. (2005). Glutamine-free media for vaccine production processes. In F. Godia, & M. Fussenegger (Eds.), Animal Cell Technology meets Genomics: Proceedings ot the 18th ESACT Meeting (pp. 767-770).

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 Urheber:
Genzel, Y.1, Autor           
Alt, R., Autor
Reichl, U.1, 2, Autor           
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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 Zusammenfassung: In cell culture technology high ammonia concentrations can limit cell growth and product formation, especially when focussing on higher cell densities. This high ammonia concentration normally results from glutamine consumption and decomposition. To overcome such problems we have tested several options and developed a glutamine-free medium for cell lines used in industrial applications. Preliminary investigations were carried out in static cultures of BHK 21, CHO and MDCK cells. Detailed characterization of MDCK cell metabolism was done for a vaccine production process of influenza virus (equine influenza Newmarket 1/93 H3N8) in MDCK cells using this newly developed glutamine-free medium: The results for cell growth and virus replication in a large-scale controlled microcarrier culture (5L bioreactor) are compared to the production in glutamine containing (2 mM) GMEM medium. Amino acid uptake and release will be compared with other on- & off-line data, to evaluate the importance of some amino acids in different phases of cell metabolism and virus replication with respect to lactate and ammonia production. © 2005 Springer. Printed in the Netherlands [accessed 2013 November 18th]

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Sprache(n): eng - English
 Datum: 2005
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 327741
DOI: 10.1007/1-4020-3103-3_159
 Art des Abschluß: -

Veranstaltung

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Titel: 18th ESACT Meeting
Veranstaltungsort: Granada, Spain
Start-/Enddatum: 2003-05-11 - 2003-05-14

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Titel: Animal Cell Technology meets Genomics : Proceedings ot the 18th ESACT Meeting
Genre der Quelle: Konferenzband
 Urheber:
Godia, F., Herausgeber
Fussenegger, M., Herausgeber
Affiliations:
-
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: - Artikelnummer: - Start- / Endseite: 767 - 770 Identifikator: -