English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Monitoring of Extracellular TCA Cycle Intermediates in Mammalian Cell Culture

Ritter, J. B., Genzel, Y., & Reichl, U. (2005). Monitoring of Extracellular TCA Cycle Intermediates in Mammalian Cell Culture. Poster presented at 19th ESACT Meeting, Harrogate, UK.

Item is

Files

show Files
hide Files
:
ESACT_2005_JR_Abstract.pdf (Any fulltext), 7KB
Name:
ESACT_2005_JR_Abstract.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: PUBLIC
License:
-

Locators

show

Creators

show
hide
 Creators:
Ritter, J. B.1, Author           
Genzel, Y.1, Author           
Reichl, U.1, 2, Author           
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

Content

show
hide
Free keywords: -
 Abstract: In recent years, monitoring of metabolites such as glucose, lactate, ammonia, glutamine, glutamate, and other amino acids has been introduced for many cell cultivations. Assaying intracellular metabolites might reveal further insights into metabolism. However, some of the mainly intracellular or even mitochondrial appearing metabolites can also be found in the medium supernatant at micromolar concentrations. Since no active transport mechanisms for excretion of these intermediates are known for most mammalian production cell lines, they might leak out of the cells and thus could be correlated to intracellular concentrations. In this work, we are investigating extracellular concentrations of five organic acids (succinic, malic, fumaric, citric, isocitric acid) during growth looking at different media and cell lines. Moreover, clear influences on concentrations were also expected to occur after viral infection of MDCK cells. The analysis is performed using an anion-exchange chromatography system (DX-320, Dionex, Idstein, Germany) with a conductivity detector. Due to high protein content of the medium, sample preparation posed some problems. Several strategies have been investigated for an optimal recovery of the abovementioned organic acids. Experiments showed that certain amounts of these organic acids already occurred in fresh medium, coming from both serum and peptone being supplements for the culture medium. Nevertheless, first results indicate significant changes of concentrations during the course of cultivation, probably not correlated to dead cells. In batch cultivation, malic, fumaric and citric acid show an almost linear increase after the lag phase, whereas isocitric and succinic acid seem to reach a constant level during stationary phase.

Details

show
hide
Language(s): eng - English
 Dates: 2005
 Publication Status: Not specified
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 227071
 Degree: -

Event

show
hide
Title: 19th ESACT Meeting
Place of Event: Harrogate, UK
Start-/End Date: 2005-05-05 - 2005-05-08

Legal Case

show

Project information

show

Source

show