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Free keywords:
optimization; multi-segment; linear gradient; resolution map; liquid chromatography
Abstract:
Based on the mechanism of chromatographic retention (the relationship between the retention of solute and the mobile phase conditions) and method of resolution map, several methods of optimizing multi-segment linear gradient elution conditions were proposed according to the different separation requirements of various samples. These methods were verified using literature data. Moreover, the advantages and disadvantages of these methods were compared. It was proved that the third method is a fast optimization method which is capable of separating all the components with relatively high resolution.
© Springer, Part of Springer Science+Business Media [accessed 2013 November 27th]
