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  Infection dynamics and virus-induced apoptosis in cell culture-based influenza vaccine production – flow cytometry and mathematical modeling

Schulze-Horsel, J., Schulze, M., Agalaridis, G., Genzel, Y., & Reichl, U. (2009). Infection dynamics and virus-induced apoptosis in cell culture-based influenza vaccine production – flow cytometry and mathematical modeling. Vaccine, 27(20), 2712-2722. doi:10.1016/j.vaccine.2009.02.027.

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Schulze-Horsel, J.1, Autor           
Schulze, M.2, Autor           
Agalaridis, G.3, Autor           
Genzel, Y.1, Autor           
Reichl, U.1, 4, Autor           
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2TU Berlin, ou_persistent22              
3Hochschule Furtwangen, University of Applied Sciences, Villingen-Schwenningen, Germany, ou_persistent22              
4Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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 Zusammenfassung: Cell culture-based influenza vaccine manufacturing is of growing importance. Depending on virus strains, differences in infection dynamics, virus-induced apoptosis, cell lysis and virus yields are observed. Comparatively little is known concerning details of virus-host cell interaction on a cellular level and virus spreading in a population of cells in bioreactors. In this study, the infection of MDCK cells with different influenza A virus strains in lab-scale microcarrier culture was investigated by flow cytometry. Together with the infection status of cells, virus-induced apoptosis was monitored. A mathematical model has been formulated to describe changes in the concentration of uninfected and infected adherent cells, dynamics of virus particle release (infectious virions, hemgglutinin content), and the time course of the percentage composition of the cell population. (C) 2009 Elsevier Ltd. All rights reserved. [accessed November 27, 2009]

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Sprache(n): eng - English
 Datum: 2009
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 399016
Anderer: 57/09
DOI: 10.1016/j.vaccine.2009.02.027
 Art des Abschluß: -

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Titel: Vaccine
Genre der Quelle: Zeitschrift
 Urheber:
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Ort, Verlag, Ausgabe: Guildford, Surrey, UK : Elsevier
Seiten: - Band / Heft: 27 (20) Artikelnummer: - Start- / Endseite: 2712 - 2722 Identifikator: ISSN: 0264-410X
CoNE: https://pure.mpg.de/cone/journals/resource/954925498091